Overview

  • Product name

  • Description

    Rabbit polyclonal to RCC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-226 of Human RCC1 (Uniprot: P18754)

  • Positive control

    • 293T, A431 and Raji whole cell lysates, Human H1299 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab152111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris- EDTA buffer pH 8.

Target

  • Function

    Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
  • Sequence similarities

    Contains 7 RCC1 repeats.
  • Post-translational
    modifications

    N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
  • Cellular localization

    Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell cycle regulatory protein antibody
    • CHC 1 antibody
    • CHC1 antibody
    • Chromosome condensation 1 antibody
    • Chromosome condensation protein 1 antibody
    • Guanine nucleotide releasing protein antibody
    • HERC2 antibody
    • Ran GEF antibody
    • RanGEF antibody
    • RCC 1 antibody
    • RCC1 antibody
    • RCC1 I antibody
    • RCC1_HUMAN antibody
    • Regulator of chromosome condensation 1 antibody
    • Regulator of chromosome condensation antibody
    • SHEP1 antibody
    • SNHG3 RCC1 antibody
    • SNHG3 RCC1 readthrough transcript antibody
    see all

Images

  • Anti-RCC1 antibody (ab152111) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human H1299 xenograft, labeling RCC1 using ab152111 at a 1/100 dilution.

References

This product has been referenced in:

  • Zhu L  et al. Surgical monotherapy may be a suitable therapeutic strategy for advanced collecting (Bellini) duct carcinoma: A case report and literature review. Exp Ther Med 12:1181-1184 (2016). IHC-P ; Human . Read more (PubMed: 27446340) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab152111.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up