Anti-RCC1 antibody (ab152156)
Key features and details
- Rabbit polyclonal to RCC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RCC1 antibody
See all RCC1 primary antibodies -
Description
Rabbit polyclonal to RCC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 2-421 of Human RCC1 (Uniprot ID P18754).
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Positive control
- Human 293T, A431, H1299, HeLa S3, HepG2, MOLT4 and Raji whole cell lysates; mouse Neuro2A and GL261 whole cell lysates/extracts; A431 cells; Human KYSE170 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly. -
Sequence similarities
Contains 7 RCC1 repeats. -
Post-translational
modificationsN-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran. -
Cellular localization
Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 1104 Human
- Entrez Gene: 100088 Mouse
- Omim: 179710 Human
- SwissProt: P18754 Human
- SwissProt: Q8VE37 Mouse
- Unigene: 469723 Human
- Unigene: 255045 Mouse
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Alternative names
- Cell cycle regulatory protein antibody
- CHC 1 antibody
- CHC1 antibody
see all
Images
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All lanes : Anti-RCC1 antibody (ab152156) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, staining RCC1 (green) using ab152156 at a 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human KYSE170 xenograft tissue, staining RCC1 using ab152156 at a 1/500 dilution.
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All lanes : Anti-RCC1 antibody (ab152156) at 1/1000 dilution
Lane 1 : Neuro2A whole cell lysate/extract
Lane 2 : GL261 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
12% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab152156 has not yet been referenced specifically in any publications.