Overview

  • Product name
  • Description
    Rabbit polyclonal to RCC1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human RCC1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P18754

  • Positive control
    • Jurkat and A431 cells. Colon carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab36760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

1/100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function
    Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
  • Sequence similarities
    Contains 7 RCC1 repeats.
  • Post-translational
    modifications
    N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
  • Cellular localization
    Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell cycle regulatory protein antibody
    • CHC 1 antibody
    • CHC1 antibody
    • Chromosome condensation 1 antibody
    • Chromosome condensation protein 1 antibody
    • Guanine nucleotide releasing protein antibody
    • HERC2 antibody
    • Ran GEF antibody
    • RanGEF antibody
    • RCC 1 antibody
    • RCC1 antibody
    • RCC1 I antibody
    • RCC1_HUMAN antibody
    • Regulator of chromosome condensation 1 antibody
    • Regulator of chromosome condensation antibody
    • SHEP1 antibody
    • SNHG3 RCC1 antibody
    • SNHG3 RCC1 readthrough transcript antibody
    see all

Images

  • Ab36760 staining human RCC1 in human colon carcinoma by immunohistochemistry using formalin fixed, paraffin embedded tissue.

References

ab36760 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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