Product nameAnti-RCC1 antibody
See all RCC1 primary antibodies
DescriptionRabbit polyclonal to RCC1
Tested applicationsSuitable for: IHC-P, IP, ICC/IF, WBmore details
Species reactivityReacts with: Human, Xenopus laevis
Recombinant full length protein (Xenopus laevis) - His-tagged and purified from E. coli.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: PBS, Whole serum
Our Abpromise guarantee covers the use of ab4785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500. Detects a band of approximately 64 kDa (predicted molecular weight: 45 kDa).|
FunctionGuanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
Sequence similaritiesContains 7 RCC1 repeats.
modificationsN-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
Cellular localizationNucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
- Information by UniProt
- Cell cycle regulatory protein antibody
- CHC 1 antibody
- CHC1 antibody
ab4785 (1/100) staining RCC1 in Skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ab4785 at a 1/500 dilution staining approximately 64kDa RCC 1 in Xenopus egg extract (lane X, 50
µg per lane) and HeLa total cell extract (lane Hu, 50 µg per lane) by western blot (ECL, 15 seconds exposure). ab4785 at a 1/500 dilution staining approximately 64kDa RCC 1 in Xenopus egg extract (lane X, 50µg per lane) and HeLa total cell extract (lane Hu, 50µg per lane) by western blot (ECL, 15 seconds exposure).
ab4785 has not yet been referenced specifically in any publications.