Product nameAnti-RCC1 antibody [EPR5857]
See all RCC1 primary antibodies
DescriptionRabbit monoclonal [EPR5857] to RCC1
Tested applicationsSuitable for: Flow Cyt, WB, IP, IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RCC1 aa 1-100. The exact sequence is proprietary.
- WB: A431, HeLa, Jurkat and 293T cell lysates. IHC-P: Human bladder carcinoma tissue. Flow Cyt: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 45 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
|ICC||1/500 - 1/1000.|
FunctionGuanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
Sequence similaritiesContains 7 RCC1 repeats.
modificationsN-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
Cellular localizationNucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
- Information by UniProt
- Cell cycle regulatory protein antibody
- CHC 1 antibody
- CHC1 antibody
All lanes : Anti-RCC1 antibody [EPR5857] (ab109379) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa
ab109379, at a 1/100 dilution, staining RCC1 in paraffin-embedded Human bladder carcinoma tissue
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RCC1 (red) with ab109379 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
This product has been referenced in:
- Li T et al. Massive renal urothelial carcinoma with renal vein tumor thrombus, pancreatic infiltration and adrenal metastasis: A case report. Oncol Lett 12:1129-1131 (2016). IHC-P ; Human . Read more (PubMed: 27446406) »
- Cekan P et al. RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence. Mol Biol Cell 27:1346-57 (2016). WB . Read more (PubMed: 26864624) »