Recombinant
RabMAb

Recombinant Anti-RCC1 antibody [EPR5859(2)] (ab181155)

Overview

  • Product name

    Anti-RCC1 antibody [EPR5859(2)]
    See all RCC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5859(2)] to RCC1
  • Host species

    Rabbit
  • Specificity

    ab181155 recognizes total RCC1 (phospho RCC1 and non-phospho RCC1), although the immunogen is phospho peptide.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RCC1 aa 1-100 (phospho S11). The exact sequence is proprietary.
    Database link: P18754

  • Positive control

    • HeLa, A431, Jurkat and 293T lysates; Human tonsil and pancreas tissues; A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/30 - 1/70.

Target

  • Function

    Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
  • Sequence similarities

    Contains 7 RCC1 repeats.
  • Post-translational
    modifications

    N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
  • Cellular localization

    Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell cycle regulatory protein antibody
    • CHC 1 antibody
    • CHC1 antibody
    • Chromosome condensation 1 antibody
    • Chromosome condensation protein 1 antibody
    • Guanine nucleotide releasing protein antibody
    • HERC2 antibody
    • Ran GEF antibody
    • RanGEF antibody
    • RCC 1 antibody
    • RCC1 antibody
    • RCC1 I antibody
    • RCC1_HUMAN antibody
    • Regulator of chromosome condensation 1 antibody
    • Regulator of chromosome condensation antibody
    • SHEP1 antibody
    • SNHG3 RCC1 antibody
    • SNHG3 RCC1 readthrough transcript antibody
    see all

Images

  • All lanes : Anti-RCC1 antibody [EPR5859(2)] (ab181155) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?

  • Western Blot analysis of RCC1 in HeLa cell lysate immunoprecipitated with ab181155 at 1/50 dilution (Lane 1). Lane 2: Negative control.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling RCC1 with ab181155 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling RCC1 with ab181155 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RCC1 with ab181155 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

References

ab181155 has not yet been referenced specifically in any publications.

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