Overview

  • Product name

  • Description

    Rabbit polyclonal to RCC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 250 and 522 of Human RCC2 (Uniprot ID: Q9P258)

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji or mouse brain whole cell lysate; SW480 xenograft.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Function

    Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
  • Sequence similarities

    Contains 7 RCC1 repeats.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA1470 antibody
    • Protein RCC2 antibody
    • RCC 2 antibody
    • RCC1 like protein TD 60 antibody
    • RCC1-like protein TD-60 antibody
    • RCC2 antibody
    • RCC2_HUMAN antibody
    • Regulator of chromosome condensation 2 antibody
    • TD60 antibody
    • Telophase disk protein of 60 kDa antibody
    see all

Images

  • All lanes : Anti-RCC2 antibody (ab154705) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 56 kDa



    7.5% SDS PAGE
  • Anti-RCC2 antibody (ab154705) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 56 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded SW480 xenograft, labeling RCC2 using ab154705 at 1/500 dilution.

References

ab154705 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154705.
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