Anti-RCC2 antibody (ab154705)
Key features and details
- Rabbit polyclonal to RCC2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RCC2 antibody
See all RCC2 primary antibodies -
Description
Rabbit polyclonal to RCC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Xenopus laevis, Zebrafish -
Immunogen
Recombinant fragment corresponding to a region within amino acids 250 and 522 of Human RCC2 (Uniprot ID: Q9P258)
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji or mouse brain whole cell lysate; SW480 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 56 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 56 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
Target
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Function
Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1. -
Sequence similarities
Contains 7 RCC1 repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex. - Information by UniProt
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Database links
- Entrez Gene: 509120 Cow
- Entrez Gene: 55920 Human
- Entrez Gene: 108911 Mouse
- Entrez Gene: 734455 Xenopus laevis
- Entrez Gene: 406455 Zebrafish
- Omim: 609587 Human
- SwissProt: Q9P258 Human
- SwissProt: Q8BK67 Mouse
see all -
Alternative names
- KIAA1470 antibody
- Protein RCC2 antibody
- RCC 2 antibody
see all
Images
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All lanes : Anti-RCC2 antibody (ab154705) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
7.5% SDS PAGE -
Anti-RCC2 antibody (ab154705) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 56 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, labeling RCC2 using ab154705 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154705 has not yet been referenced specifically in any publications.