Overview

  • Product name

  • Description

    Rabbit polyclonal to RCC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human RCC2 (internal sequence). The exact sequence is proprietary.
    Database link: Q9P258

  • Positive control

    • WB: Mouse brain tissue lysate; MCF7 whole cell lysate. ICC/IF: MCF7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
  • Sequence similarities

    Contains 7 RCC1 repeats.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA1470 antibody
    • Protein RCC2 antibody
    • RCC 2 antibody
    • RCC1 like protein TD 60 antibody
    • RCC1-like protein TD-60 antibody
    • RCC2 antibody
    • RCC2_HUMAN antibody
    • Regulator of chromosome condensation 2 antibody
    • TD60 antibody
    • Telophase disk protein of 60 kDa antibody
    see all

Images

  • Paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for RCC2 (green) using ab229153 at 1/500 dilution in immunohistochemical analysis.

  • Anti-RCC2 antibody (ab229153) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg

    Predicted band size: 56 kDa



    7.5% SDS-PAGE gel.

  • Anti-RCC2 antibody (ab229153) at 1/2000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 56 kDa



    7.5% SDS-PAGE gel.

References

ab229153 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229153.
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