Key features and details
- Rabbit polyclonal to RCC2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RCC2 antibody
See all RCC2 primary antibodies
DescriptionRabbit polyclonal to RCC2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Dog, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide (Human) corresponding to to a region between residues 1 and 50 of human RCC2. (NP_061185.1)
- Whole cell lysate from HeLa and 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).|
|IP||Use a concentration of 2 - 5 µg/ml.|
FunctionRequired for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
Sequence similaritiesContains 7 RCC1 repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.
- Information by UniProt
- KIAA1470 antibody
- Protein RCC2 antibody
- RCC 2 antibody
All lanes : Anti-RCC2 antibody (ab70787) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 56 kDa
Observed band size: 56 kDa
HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab70787 at 1 µg/ml for WB, and for IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab70787 has not yet been referenced specifically in any publications.