Anti-RCC2 antibody (ab70787)
Key features and details
- Rabbit polyclonal to RCC2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RCC2 antibody
See all RCC2 primary antibodies -
Description
Rabbit polyclonal to RCC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Dog, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide (Human) corresponding to to a region between residues 1 and 50 of human RCC2. (NP_061185.1)
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Positive control
- Whole cell lysate from HeLa and 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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IP |
Use a concentration of 2 - 5 µg/ml.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
IP
Use a concentration of 2 - 5 µg/ml. |
Target
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Function
Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1. -
Sequence similarities
Contains 7 RCC1 repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex. - Information by UniProt
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Database links
- Entrez Gene: 509120 Cow
- Entrez Gene: 55920 Human
- Entrez Gene: 108911 Mouse
- Omim: 609587 Human
- SwissProt: Q9P258 Human
- SwissProt: Q8BK67 Mouse
- Unigene: 380857 Human
- Unigene: 253 Mouse
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Alternative names
- KIAA1470 antibody
- Protein RCC2 antibody
- RCC 2 antibody
see all
Images
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All lanes : Anti-RCC2 antibody (ab70787) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 56 kDa
Observed band size: 56 kDa -
HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab70787 at 1 µg/ml for WB, and for IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab70787 has been referenced in 1 publication.
- Xu X et al. MicroRNA-191 modulates cisplatin-induced DNA damage response by targeting RCC2. FASEB J 34:13573-13585 (2020). PubMed: 32803782