Key features and details
- Rabbit polyclonal to RCC2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RCC2 antibody
See all RCC2 primary antibodies
DescriptionRabbit polyclonal to RCC2
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant
Synthetic peptide corresponding to a region between residue 475 and the C-terminus (residue 522) of human RCC2. (NP_061185.1).
- HeLa and 293T whole cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).|
FunctionRequired for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
Sequence similaritiesContains 7 RCC1 repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.
- Information by UniProt
- KIAA1470 antibody
- Protein RCC2 antibody
- RCC 2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RCC2 with ab70788 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-RCC2 antibody (ab70788) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 56 kDa
Observed band size: 56 kDa
ab70788 has been referenced in 1 publication.
- Atkinson SJ et al. The ß3-integrin endothelial adhesome regulates microtubule-dependent cell migration. EMBO Rep 19:N/A (2018). PubMed: 29794156