Anti-RCE1 antibody (ab62531)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-RCE1 antibody
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DescriptionRabbit polyclonal to RCE1
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
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Immunogen
A region within synthetic peptide: WARCLTDMRW LRNQVIAPLT EELVFRACML PMLAPCMGLG PAVFTCPLFF, corresponding to amino acids 155-204 of Human RCE1
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Positive control
- HepG2 cell lysate. Human muscle tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab62531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| ELISA | Use at an assay dependent concentration. Titre using peptide based assay: 1/312500. |
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| WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 4 - 8 µg/ml. |
Target
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RelevanceRCE1 (RCE1 homolog, prenyl protein peptidase) is a member of the metalloproteinase family. It functions in the maintenance and processing of CAAX-type prenylated proteins, proteolytically removing the C-terminal three residues of farnesylated and geranylated proteins.
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Cellular localizationEndoplasmic reticulum membrane; Multi-pass membrane protein.
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Database links
- Entrez Gene: 539539 Cow
- Entrez Gene: 483705 Dog
- Entrez Gene: 100861127 Goat
- Entrez Gene: 9986 Human
- Entrez Gene: 19671 Mouse
- Omim: 605385 Human
- SwissProt: A6H7A0 Cow
- SwissProt: Q9Y256 Human
see all -
Alternative names
- CAAX prenyl protease 2 antibody
- FACE 2 antibody
- FACE2 antibody
see all
Images
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Western blot - Anti-RCE1 antibody (ab62531)All lanes : Anti-RCE1 antibody (ab62531) at 1.25 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : HepG2 cell lysate at 10 µg
Secondary
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Gel concentration: 12% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCE1 antibody (ab62531)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling RCE1 with ab62531 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.
Datasheets and documents
References
ab62531 has not yet been referenced specifically in any publications.
