Product nameAnti-RCE1 antibody
DescriptionRabbit polyclonal to RCE1
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
- HepG2 cell lysate. Human muscle tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab62531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
RelevanceRCE1 (RCE1 homolog, prenyl protein peptidase) is a member of the metalloproteinase family. It functions in the maintenance and processing of CAAX-type prenylated proteins, proteolytically removing the C-terminal three residues of farnesylated and geranylated proteins.
Cellular localizationEndoplasmic reticulum membrane; Multi-pass membrane protein.
- CAAX prenyl protease 2 antibody
- FACE 2 antibody
- FACE2 antibody
All lanes : Anti-RCE1 antibody (ab62531) at 1.25 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : HepG2 cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling RCE1 with ab62531 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.
ab62531 has not yet been referenced specifically in any publications.