Overview

  • Product name
    Anti-RCE1 antibody
  • Description
    Rabbit polyclonal to RCE1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: WARCLTDMRW LRNQVIAPLT EELVFRACML PMLAPCMGLG PAVFTCPLFF, corresponding to amino acids 155-204 of Human RCE1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HepG2 cell lysate. Human muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    RCE1 (RCE1 homolog, prenyl protein peptidase) is a member of the metalloproteinase family. It functions in the maintenance and processing of CAAX-type prenylated proteins, proteolytically removing the C-terminal three residues of farnesylated and geranylated proteins.
  • Cellular localization
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • Database links
    see all
  • Alternative names
    • CAAX prenyl protease 2 antibody
    • FACE 2 antibody
    • FACE2 antibody
    • Farnesylated protein converting enzyme 2 antibody
    • Farnesylated proteins converting enzyme 2 antibody
    • hRCE 1 antibody
    • hRCE1 antibody
    • Prenyl protein peptidase RCE1 antibody
    • Prenyl protein specific endoprotease 2 antibody
    • RCE 1 antibody
    • RCE 1A antibody
    • RCE 1B antibody
    • RCE1 homolog prenyl protein peptidase antibody
    • RCE1A antibody
    • RCE1B antibody
    see all

Images

  • Western blot - Anti-RCE1 antibody (ab62531)
    Western blot - Anti-RCE1 antibody (ab62531)
    All lanes : Anti-RCE1 antibody (ab62531) at 1.25 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 36 kDa
    Observed band size: 34 kDa (why is the actual band size different from the predicted?)



    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCE1 antibody (ab62531)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCE1 antibody (ab62531)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling RCE1 with ab62531 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

References

ab62531 has not yet been referenced specifically in any publications.

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