Overview

  • Product name

    Anti-RCL antibody [EPR8685]
    See all RCL primary antibodies
  • Description

    Rabbit monoclonal [EPR8685] to RCL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RCL. The exact sequence is proprietary.

  • Positive control

    • K562, Jurkat, HepG2 and 293T cell lysates, Human breast carcinoma and Human kidney tissues
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 19 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases.
  • Tissue specificity

    Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers.
  • Sequence similarities

    Belongs to the deoxyribonucleoside 5'-monophosphate N-glycosidase family.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2' deoxynucleoside 5' phosphate N hydrolase 1 antibody
    • BC048355 antibody
    • c Myc responsive antibody
    • c Myc responsive protein Rcl antibody
    • c-Myc-responsive protein Rcl antibody
    • C6orf108 antibody
    • C76683 antibody
    • Chromosome 6 open reading frame 108 antibody
    • Deoxyribonucleoside 5' monophosphate N glycosidase antibody
    • Deoxyribonucleoside 5''-monophosphate N-glycosidase antibody
    • dJ330M21.3 antibody
    • DNPH1 antibody
    • MGC54855 antibody
    • Putative c Myc responsive antibody
    • Rcl antibody
    • RCL_HUMAN antibody
    • RP3 330M21.3 antibody
    see all

Images

  • All lanes : Anti-RCL antibody [EPR8685] (ab150413) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 19 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RCL with ab150413 antibody at a dilution of 1/100.
  • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling RCL with ab150413 antibody at a dilution of 1/100.

References

ab150413 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab150413.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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