Recombinant Anti-RCL antibody [EPR8686] (ab131252)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RCL antibody [EPR8686]
See all RCL primary antibodies -
Description
Rabbit monoclonal [EPR8686] to RCL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RCL aa 150-250. The exact sequence is proprietary.
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Positive control
- Human breast carcinoma tissue; K562, HepG2, HeLa, Jurkat and MCF7 cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8686 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 19 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | 1/100 - 1/250. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases. -
Tissue specificity
Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers. -
Sequence similarities
Belongs to the deoxyribonucleoside 5'-monophosphate N-glycosidase family. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10591 Human
- SwissProt: O43598 Human
- Unigene: 109752 Human
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Alternative names
- 2' deoxynucleoside 5' phosphate N hydrolase 1 antibody
- BC048355 antibody
- c Myc responsive antibody
see all
Images
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![Western blot - Anti-RCL antibody [EPR8686] (ab131252)](https://www.abcam.com/ps/products/131/ab131252/Images/ab131252-145573-anti-rcl-antibody-epr8686-western-blot.jpg)
Western blot - Anti-RCL antibody [EPR8686] (ab131252)All lanes : Anti-RCL antibody [EPR8686] (ab131252) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 19 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCL antibody [EPR8686] (ab131252)](https://www.abcam.com/ps/products/131/ab131252/Images/ab131252-145572-anti-rcl-antibody-epr8686-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCL antibody [EPR8686] (ab131252)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RCL with ab131252 antibody at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Other - Anti-RCL antibody [EPR8686] (ab131252)](https://www.abcam.com/ps/products/131/ab131252/Images/ab131252-145571-anti-rcl-antibody-epr8686.jpg)
Other - Anti-RCL antibody [EPR8686] (ab131252)
Protocols
Datasheets and documents
References
ab131252 has not yet been referenced specifically in any publications.
