Recombinant
RabMAb

Recombinant Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)

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Overview

  • Product name

    Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free
    See all RCN1/RCN primary antibodies

  • Description

    Rabbit monoclonal [EPR17163] to RCN1/RCN - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human RCN1/RCN aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15293

  • Positive control

    • IHC: Human cerebral cortex tissue.ICC/IF: HeLa cells.IP and FC: HeLa cells.

  • General notes

    ab246331 is the carrier-free version of ab198996 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab246331 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as RCN1

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17163

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab246331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 44, 46 kDa (predicted molecular weight: 39 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

Images

  • Flow Cytometry - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)
    Flow Cytometry - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)

    Flow cytometry analysis of HeLa cells labelling RCN1/RCN (red) with purified ab198996 at dilution of 1/70. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198996).

  • Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)
    Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell from cervix adenocarcinoma) cells labeling RCN1/RCN with ab198996 at 1/250, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000  and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:-
    -ve control 1 - ab198996 at 1/250 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198996).

  • Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)
    Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (Human breast adenocarcinoma) cells labeling RCN1/RCN with ab198996 at 1/250, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF-7 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab198996 at 1/250 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198996).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling RCN1/RCN with ab198996 at 1/1600, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Human cerebral cortex tissue is observed. Subcellular location Endoplasmic reticulum lumen [UniProt]. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198996).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)
    Immunoprecipitation - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)

    RCN1/RCN was immunoprecipitated from 1mg of HeLa  (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab198996 at 1/100. Western blot was performed from the immunoprecipitate using ab198996 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract. 10ug (Input.

    Lane 2: HeLa whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198996 in HeLa whole cell extract.

    The expression profile observed is consistent with what has been described in the literature PMID: 8416973. 

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198996).

     

  • Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)
    Immunocytochemistry/ Immunofluorescence - Anti-RCN1/RCN antibody [EPR17163] - BSA and Azide free (ab246331)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling RCN1/RCN with ab198996 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198996).

References

ab246331 has not yet been referenced specifically in any publications.

Publishing research using ab246331? Please let us know so that we can cite the reference in this datasheet.

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