Recombinant
RabMAb

Recombinant Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996)

Overview

  • Product name

    Anti-RCN1/RCN antibody [EPR17163] - C-terminal
    See all RCN1/RCN primary antibodies
  • Description

    Rabbit monoclonal [EPR17163] to RCN1/RCN - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human RCN1/RCN aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15293

  • Positive control

    • WB: 293 cell lysate and HeLa cell lysate. IHC: Human cerebral cortex tissue. ICC/IF: HeLa cells. IP and FC: HeLa cells.
  • General notes

     

     

     This product was previously labelled as RCN1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17163
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab198996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/70.
IHC-P 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 44, 46 kDa (predicted molecular weight: 39 kDa).
ICC/IF 1/250.
IP 1/100.

Target

Images

  • All lanes : Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/10000 dilution

    Lane 1 : 293 (Human epithelial cells from embryonic kidney) cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 44, 46 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking and diluting buffer was 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature PMID: 8416973.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling RCN1/RCN with ab198996 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling RCN1/RCN with ab198996 at 1/1600, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Human cerebral cortex tissue is observed. Subcellular location Endoplasmic reticulum lumen [UniProt]. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/1000 dilution

    Lane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 44, 46 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking and diluting buffer was 5% NFDM/TBST.
    The expression profile observed is consistent with what has been described in the literature PMID: 8416973.

  • RCN1/RCN was immunoprecipitated from 1mg of HeLa  (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab198996 at 1/100. Western blot was performed from the immunoprecipitate using ab198996 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract. 10ug (Input.

    Lane 2: HeLa whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198996 in HeLa whole cell extract.

    The expression profile observed is consistent with what has been described in the literature PMID: 8416973. 

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

  • Flow cytometry analysis of HeLa cells labelling RCN1/RCN (red) with purified ab198996 at dilution of 1/70. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

References

ab198996 has not yet been referenced specifically in any publications.

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