Recombinant Anti-RCN1/RCN antibody [EPR19193] (ab184441)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19193] to RCN1/RCN
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RCN1/RCN antibody [EPR19193]
See all RCN1/RCN primary antibodies -
Description
Rabbit monoclonal [EPR19193] to RCN1/RCN -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver, heart, kidney and spleen lysates; HeLa, HEK-293, U-87 MG, C6, SH-SY5Y, Neuro-2a, RAW 264.7, PC-12 and NIH/3T3 cell lysates; E14 mouse and rat embryo lysates; Rat cerebral cortex, brain, heart and spleen lysates; Mouse brain, heart and spleen lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19193 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Detects a band of approximately 44, 46 kDa (predicted molecular weight: 38 kDa).
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Notes |
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WB
1/2000. Detects a band of approximately 44, 46 kDa (predicted molecular weight: 38 kDa). |
Target
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Function
May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. -
Sequence similarities
Belongs to the CREC family.
Contains 6 EF-hand domains. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 5954 Human
- Entrez Gene: 19672 Mouse
- Entrez Gene: 362182 Rat
- Omim: 602735 Human
- SwissProt: Q15293 Human
- SwissProt: Q05186 Mouse
- Unigene: 97887 Human
- Unigene: 397500 Mouse
see all -
Alternative names
- Epididymis secretory protein Li 84 antibody
- FLJ37041 antibody
- HEL S 84 antibody
see all
Images
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All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 44,46 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1, 2 and 3: 30 seconds; Lane 4: 3 minutes.
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All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : E14 mouse embryo lysate
Lane 5 : E14 rat embryo lysate
Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 7 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 8 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 9 : Rat cerebral cortex lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 44,46 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-6: 8 seconds; Lane 7 and 8: 30 seconds; Lane 9: 3 minutes.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8416973).
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All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lane 6 : Rat spleen lysate
Lane 7 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 10 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 44,46 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-6: 3 minutes Lane 7 and 8: 30 seconds; Lane 9 and 10: 1 second.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab184441 has been referenced in 3 publications.
- Fu H et al. Reticulocalbin 1 is required for proliferation and migration of non-small cell lung cancer cells regulated by osteoblast-conditioned medium. J Cell Mol Med 25:11198-11211 (2021). PubMed: 34747128
- Huang ZH et al. Reticulocalbin-1 knockdown increases the sensitivity of cells to Adriamycin in nasopharyngeal carcinoma and promotes endoplasmic reticulum stress-induced cell apoptosis. Cell Cycle 19:1576-1589 (2020). PubMed: 32436770
- Chen X et al. Overexpression of RCN1 correlates with poor prognosis and progression in non-small cell lung cancer. Hum Pathol 83:140-148 (2019). PubMed: 30172915