Overview

  • Product name
  • Description
    Rabbit polyclonal to RDH10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide within Human RDH10 aa 113-143 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8IZV5

  • Positive control
    • NCI-H460 cells. Human lung carcinoma tissue. WiDr, NCI-H460 and HepG2 cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab174340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 38 kDa.

Target

Images

  • Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human lung carcinoma tissue labeling RDH10 with ab174340 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • All lanes : Anti-RDH10 antibody (ab174340) at 1/100 dilution

    Lane 1 : WiDr cell line lysates
    Lane 2 : NCI-H460 cell line lysates
    Lane 3 : HepG2 cell line lysates

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 38 kDa

  • Flow Cytometric analysis of NCI-H460 cells labeling RDH10 with ab174340 at 1/10 dilution.

References

This product has been referenced in:
  • Guan F  et al. Retinol dehydrogenase-10 promotes development and progression of human glioma via the TWEAK-NF-?B axis. Oncotarget 8:105262-105275 (2017). Read more (PubMed: 29285249) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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