Overview

  • Product name

  • Description

    Rabbit polyclonal to RDH5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 110-246 of Human RDH5 (AAH28298).

  • Positive control

    • NT2D1 whole cell lysate; A549, HeLa and Jurkat cell lysates; Cal27 xenograft

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer or Tris-EDTA buffer (pH8.0)

Target

  • Relevance

    RDH5 encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments.
  • Cellular localization

    Membrane; Peripheral membrane protein
  • Database links

  • Alternative names

    • 11 cis RDH antibody
    • 11 cis retinol dehydrogenase antibody
    • 9 cis retinol specific dehydrogenase antibody
    • FLJ39337 antibody
    • FLJ97089 antibody
    • HSD17B9 antibody
    • RDH 5 antibody
    • RDH1 antibody
    • Retinol dehydrogenase 1 antibody
    • Retinol dehydrogenase 5 (11 cis/9 cis) antibody
    • Retinol dehydrogenase 5 antibody
    • SDR9C5 antibody
    • Short chain dehydrogenase/reductase family 9C member 5 antibody
    see all

Images

  • Anti-RDH5 antibody (ab101457) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size: 35 kDa



    10% SDS-PAGE
  • Immunohistochemical analysis of RDH5 in paraffin-embedded Cal27 xenograft, using ab101457 at 1/100 dilution.

References

This product has been referenced in:

  • Samuel W  et al. Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells. Mol Vis 23:60-89 (2017). Human . Read more (PubMed: 28356702) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting Abcam this morning.

As we talked about on the phone this morning, I am sending you ab106738 as a free of charge replacement, so that you can see if you still get the same molecular weight band as you did when using ab101457. Your new order number is ****** .

Please let me know if there is anything else I can help you with.

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