Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab106738 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab106738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 35 kDa.|
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceRDH5 encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments.
Cellular localizationMembrane; Peripheral membrane protein
- 11 cis RDH antibody
- 11 cis retinol dehydrogenase antibody
- 9 cis retinol specific dehydrogenase antibody
ab106738 (5µg/ml) staining of RDH5 in paraffin embedded Human Retina following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows staining of the Ganglion Cell Layer.
Anti-RDH5 antibody (ab106738) at 2 µg/ml + Mouse Eye Lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Primary incubation was 1 hour.
ab106738 has not yet been referenced specifically in any publications.