Anti-REA antibody (ab71970)
Key features and details
- Rabbit polyclonal to REA
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-REA antibody
See all REA primary antibodies -
Description
Rabbit polyclonal to REA -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human REA.
Database link: Q99623 -
Positive control
- WB: HeLa, HEK-293T, NIH/3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IP |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
Target
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Function
Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. -
Sequence similarities
Belongs to the prohibitin family. -
Developmental stage
Levels of expression in fibroblasts decrease heterogeneously during cellular aging. -
Cellular localization
Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear. - Information by UniProt
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Database links
- Entrez Gene: 515363 Cow
- Entrez Gene: 11331 Human
- Entrez Gene: 12034 Mouse
- Entrez Gene: 114766 Rat
- Omim: 610704 Human
- SwissProt: Q2HJ97 Cow
- SwissProt: Q99623 Human
- SwissProt: O35129 Mouse
see all -
Alternative names
- B cell receptor associated protein BAP37 antibody
- B-cell receptor-associated protein BAP37 antibody
- BAP antibody
see all
Images
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All lanes : Anti-REA antibody (ab71970) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands. -
ICC/IF image of ab71970 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71970, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab71970 has been referenced in 3 publications.
- Toki S et al. The survival and proliferation of osteosarcoma cells are dependent on the mitochondrial BIG3-PHB2 complex formation. Cancer Sci 112:4208-4219 (2021). PubMed: 34363714
- Sircoulomb F et al. ZNF703 gene amplification at 8p12 specifies luminal B breast cancer. EMBO Mol Med 3:153-66 (2011). ICC/IF ; Human . PubMed: 21328542
- He B et al. A repressive role for prohibitin in estrogen signaling. Mol Endocrinol 22:344-60 (2008). WB, IP ; Mouse . PubMed: 17932104