Actin filament polymerization: molecular targets

Explore our range of products to study the actin assembly process.

The dynamic nature of actin polymerization and disassembly is central to functions such as cell adhesion and migration. For this reason, molecular mechanisms of actin reorganization are under investigation for therapeutics targeting cancer and cardiovascular diseases.​

We have highlighted some key actin nucleators, regulatory proteins and toxins that can help you study the actin polymerization process at different stages.

·         Nucleation factors

·         Regulatory co- factors​

·         Toxins

·         Visualization tools


Nucleation factors

Target protein

Function

Marker

Inhibitor

Formin

Nucleation and elongation factors that promote linear actin filaments

Anti-FMNL2 antibody

Anti-DIAPH1 antibody 

SMIFH2


ARP 2/3 complex

Mimics actin trimer and acts as a nucleation site to form branched actin filaments (see Figure 1)

Anti-ARPC2 antibody 

Anti-Arp2 antibody 

Anti-Arp3 antibody 

CK666 

WASp

Regulator of the Arp2/3 complex

Anti-N WASP antibody 

Wiskostatin 

Spires

Organizes up actin monomers into prenucleation scaffold for polymerization

Anti-SPIRE1 antibody 














Regulatory co- factors 

Target protein

Function

Marker

Protein/peptide


Thymosin β4

G-actin-sequestering

protein which allows filament polymerization

Anti-Thymosin beta 4 antibody 

Human Thymosin beta 4 peptide 

Profilin

Facilitates formation of ATP-actin monomers, which are then available for polymerization

Anti-Profilin 1 antibody 

Recombinant Human Profilin 1 protein 


Cofilin

Increases dissociation of actin monomers from filaments causing depolymerization

Anti-Cofilin antibody 

Anti-Cofilin (phospho S3) antibody 

Recombinant Human Cofilin protein 

Human Cofilin peptide 











Toxins



Toxin name






Function






Small molecule

Phalloidin      

Binds tightly to actin filaments and prevents their dissociation (depolymerization inhibitor)

Phalloidin 

Latrunculin

Binds to actin monomers to inhibit polymerization - promotes filament disassembly

Latrunculin A 

Latrunculin B 

Cytochalasin

Binds to the plus ends of actin filaments and prevent filament assembly and disassembly

Cytochalasin A,  Cytochalasin B,

Cytochalasin C, Cytochalasin D,

Cytochalasin E

Jasplakinolide

Stabilizes actin monomers, and inducer of actin polymerization

Jasplakinolide 

Chaetoglobosin A

Inhibits actin polymerization

Chaetoglobosin A 


Actin visualization tools

Target protein

Tool

Alpha actin

Anti-alpha smooth muscle Actin

Conjugated versions

Beta actin

Anti-beta Actin antibody [AC-15]

Conjugated versions

F- actin

CytoPainter Phalloidin

F- actin CytoPainter F-actin Staining Kit

Figure 1. CytoPainter F-actin Staining Kit - Blue Fluorescence (ab112124).  F-actin was stained with CytoPainter blue in mouse embryonic stem cell-differentiated embryoid bodies using ICC/IF. Image courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School. 

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