For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Assay development for drug discovery

Related

  • Improving efficiency in drug discovery and development
    • Customized products
      • Complete guide to multiplex assays in drug discovery
        • Find solutions to reduce risk in your workflow
          • See how we can work with you to help you succeed

            In-depth review of key factors in assay development that can be significantly influenced by choice of reagents and assay format. 

            Published 29th April 2022

            Download the guide here.

            Introduction

            High-quality, robust assays are the foundation for successful drug discovery and development. The choices you make during assay development for high-throughput screening (HTS) can profoundly influence everything from the classes of target you can identify to the quality of the hits and leads you progress, to the success of your application for a new drug (NDA) or biologics license (BLA).

            Are the antibodies you will use in your assay sensitive and specific enough given the available sample volumes and expected target molecule concentrations? Are there any components in the sample or reaction mix that could interfere with the assay? How will the formulation and quality of a particular reagent affect assay performance? Numerous questions, such as these, need to be considered during assay development to avoid compromising data quality and introducing problems that could seriously delay or derail your project.

            In this guide, we explore the key factors in assay development that can be significantly influenced by choice of reagents and assay format.

            The guide covers the following topics:

            • Tracking assay performance
            • Sample matrix effects
            • Z' factor as a metric of assay quality
            • Factors influencing assay quality
            • Considerations for creating a high-quality HTS assay
            • Selecting reagents for drug discovery and HTS assays
            • How Abcam can help with assay development

            Download our Assay development guide for drug discovery here.

            How Abcam can help with assay development

            • Recombinant antibodies
            • Carrier-free, conjugation-ready antibodies, conjugation kits and other conjugation solutions
            • Matched antibody pairs
            • Knock-out cell lines
            • Multiplex high-throughput immunoassays
            • Proteins and peptides





            Get resources and offers direct to your inbox Sign up
            A-Z by research area
            • Cancer
            • Cardiovascular
            • Cell biology
            • Developmental biology
            • Epigenetics & Nuclear signaling
            • Immunology
            • Metabolism
            • Microbiology
            • Neuroscience
            • Signal transduction
            • Stem cells
            A-Z by product type
            • Primary antibodies
            • Secondary antibodies
            • Biochemicals
            • Isotype controls
            • Flow cytometry multi-color selector
            • Kits
            • Loading controls
            • Lysates
            • Peptides
            • Proteins
            • Slides
            • Tags and cell markers
            • Tools & Reagents
            Help & support
            • Support
            • Make an Inquiry
            • Protocols & troubleshooting
            • Placing an order
            • RabMAb products
            • Biochemical product FAQs
            • Training
            • Browse by Target
            Company
            • Corporate site
            • Investor relations
            • Company news
            • Careers
            • About us
            • Blog
            Events
            • Tradeshows
            • Conferences
            International websites
            • abcam.cn
            • abcam.co.jp

            Join with us

            • LinkedIn
            • facebook
            • Twitter
            • YouTube
            • Terms of sale
            • Website terms of use
            • Cookie policy
            • Privacy policy
            • Legal
            • Modern slavery statement
            © 1998-2022 Abcam plc. All rights reserved.