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In-depth review of key factors in assay development that can be significantly influenced by choice of reagents and assay format.
Published 29th April 2022
High-quality, robust assays are the foundation for successful drug discovery and development. The choices you make during assay development for high-throughput screening (HTS) can profoundly influence everything from the classes of target you can identify to the quality of the hits and leads you progress, to the success of your application for a new drug (NDA) or biologics license (BLA).
Are the antibodies you will use in your assay sensitive and specific enough given the available sample volumes and expected target molecule concentrations? Are there any components in the sample or reaction mix that could interfere with the assay? How will the formulation and quality of a particular reagent affect assay performance? Numerous questions, such as these, need to be considered during assay development to avoid compromising data quality and introducing problems that could seriously delay or derail your project.
In this guide, we explore the key factors in assay development that can be significantly influenced by choice of reagents and assay format.
The guide covers the following topics:
How Abcam can help with assay development