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Explore the power of knock-out cell lines for your research

COVID-19 research: knock-out (KO) cell lines and lysates

Related

  • COVID-19 product and information hub
    • Maximise efficiency on returning to the lab
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        • Knock-out (KO) cell lines
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              • Fast track your research with KO cell lines
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                        • Knock-out (KO) cell lysates
                          • Five pillars to determine antibody specificity

                            Save time with ready-made cell engineering products so you can get on with answering the bigger research questions. 

                            Published 8th March 2021

                            Knock-out cell lines and lysates are essential reagents in understanding and further supporting the important research into COVID-19. Below is a curated list of high-quality, in-house manufactured knock-out products that enable you to confidently interrogate the relationship between genotype and phenotype without the complexity of creating gene-edited cell lines yourself. 

                            Our ever-expanding catalog of COVID-19 related ready-made knock-out cell lines and lysates allows you to get your knock-out of choice in less than five days. All are easily accessible, increasingly relevant, and expertly made, providing you with a hassle-free solution. 

                            Let Abcam's gene-edited cell lines help you get on with answering the bigger research questions. 

                            ​​COVID-19 related knock-outs


                            Target and relation to COVID-19ImageKO cell lineKO cell lysate

                            ACE2

                            ACE2 protects the lung and several other tissues from injury, given its role as a regulator of the renin-angiotensin system. It also acts as the cellular receptor for both SARS-CoV and SARS-CoV-2 (COVID-19). Due to the key role that ACE2 plays in host cell invasion by SARS-CoV-2, strategies are underway to develop drugs that can block this target.

                            ​

                            Human ACE2 knock-out Caco 2 cell line (ab273731)

                            Human ACE2 knock-out Caco 2 cell lysate (ab275516)

                            Human ACE2 knock-out HepG2 cell line (ab273733)

                            Human ACE2 knock-out HepG2 cell lysate (ab275495)

                            ANPEP / CD13

                            Increased expression of the ectopeptidatase ANPEP/CD13 is a hallmark of inflammation. Therefore reduction of ANPEP activity has been investigated as a possible anti‐inflammatory therapy. ANPEP is a known receptor for coronaviruses, among them SARS-CoV-2 (COVID-19). Elevated ANPEP levels may contribute to the severity of SARS‐CoV‐2 infection and it has been speculated that it may be involved in the development of thrombocytopenia in patients with COVID-19. 


                            ​

                            Human ANPEP (CD13) knock-out THP-1 cell line (ab273759)

                            Human ANPEP (CD13) knock-out THP-1 cell lysate (ab275505)

                            CD147 / BSG

                            CD147, known as basigin or EMMPRIN, is a transmembrane glycoprotein involved in tumor development, and bacterial and virus infection. CD147 acts as a receptor for SARS-CoV-2 entry. Loss of CD147 or blocking CD147 by Meplazumab, an anti-CD147 humanized antibody, inhibits SARS-CoV-2 replication, whereas CD147 overexpression promotes virus infection, thus highlighting the potential of inhibiting CD147 as a target for COVID-19 treatment. 

                            ​​

                            Human BSG (CD147) knock-out A549 cell line (ab273748)

                            Human BSG (CD147) knock-out A549 cell lysate (ab275500)

                            CD74

                            Macrophage migration inhibitory factor (MIF) is a critical mediator of the host defense and is involved in both acute and chronic responses in the lung. CD74 forms part of the MIF receptor complex and it induces p44/p42 MAPK activation and MIF-mediated pulmonary inflammation. CD74 contributes to the adaptive immunity impairment in patients with COVID-19 and hence, CD74 and MIF may be potential targets to decrease neutrophilic lung inflammation and acute lung injury. Moreover, higher serum soluble CD74 levels may reflect the severity of lung injury and help physicians determine the prognosis of acute respiratory distress syndrome.

                            ​

                            Human CD74 knock-out Raji cell line (ab273378)

                            Human CD74 knock-out Raji cell lysate (ab275529)

                            CXCR5

                            CXCR5 is a major chemokine receptor mainly expressed on the surface of B cells and a subset of T cells. It binds to B-lymphocyte chemoattractant CXCL13 and is required for B cell homing. CXCL13 is found at a higher serum level in the severe COVID-19 patients than in the non-severe patients. CXCR5 expression is reduced on all major B cell subsets and non-naïve CD4 T cells in COVID-19 patients.

                            ​

                            Human CXCR5 knock-out Raji cell line (ab273380)

                            Human CXCR5 knock-out Raji cell lysate (ab275531)

                            IL-1 beta

                            High levels of IL-1B together with IL-6 and IL-10 have been shown to drive airway inflammation in acute respiratory distress syndrome observed in some patients with COVID-19.

                            ​

                            Human IL1B knock-out THP-1 cell line (ab273762)

                            Human IL1B knock-out THP-1 cell lysate (ab275508)

                            IL-13 receptor alpha 2

                            IL13RA2 encodes the alpha-2 receptor subunit for interleukin-13 (IL-13). The IL-13 pathway has immunoregulatory functions and is implicated in several lung diseases. The IL-13 pathway can activate Janus kinase 2 (JAK2) and it has been shown that inhibition of JAK2 blocks SARS-CoV-2 viral entry.

                            ​

                            Human IL13RA2 knock-out A375 cell line (ab273381)

                            Human IL13RA2 knock-out A375 cell lysate (ab275532)


                            IL1RAP 

                            IL1RAP is required to activate IL-1 receptor signaling which is highly upregulated in patients with severe COVID-19 disease.​

                            ​

                            Human IL1RAP knock-out HeLa cell line (ab273375)

                            Human IL1RAP knock-out HeLa cell lysate (ab275526)

                            IL-6

                            Systemic inflammation and respiratory failure in patients with COVID-19 are associated with increased release of cytokines, including IL-6. IL-6 may be used as a marker to predict respiratory failure, thus helping physicians correctly allocate patients at an early stage. Moreover, it is hypothesized that modulating the levels of IL-6 or its effects may alter the course of COVID-19. 


                            ​

                            Human IL-6 knock-out A549 cell line (ab273751)

                            Human IL-6 knock-out A549 cell lysate (ab275501)

                            CXCL8 / IL-8

                            IL-8 has a potent role in the recruitment and activation of neutrophils, often elevated in patients with COVID-19. IL-8 is produced by lung epithelial cells upon infection and it is found to be highly expressed in patients with severe COVID-19 infection.

                            ​

                            Human CXCL8 (IL-8) knock-out PC3 cell line (ab273743)

                            Human CXCL8 (IL-8) knock-out PC3 cell lysate (ab275520)

                            CCL7 / MCP3

                            Bronchoalveolar fluid from patients with severe COVID-19 is enriched in CCL2 and CCL7, two chemokines that are the most potent at recruiting CCR2+ monocytes. Continuously high levels of CXCL10, CCL7, and IL-1 receptor antagonist cytokines in plasma of patients with COVID-19 are associated with increased viral load, loss of lung function, lung injury, and a fatal outcome. 

                            ​

                            Mouse CCL7 knock-out RAW 264.7 cell line (ab273755)

                            Mouse CCL7 knock-out RAW 264.7 cell lysate (ab275525)

                            TNF alpha

                            Increased inflammatory cytokines such as TNFα and IL-6 are observed in patients with COVID-19, especially in the severe group. Elevated IL-6 and TNF-α serum levels at presentation are strong predictors of disease severity and survival.


                            ​​

                            Human TNF knock-out THP-1 cell line (ab273761)

                            Human TNF knock-out THP-1 cell lysate (ab275507)

                            Want to see more exciting knock-out cell lines and lysates?

                            View our full range of new cell engineering products and filter on your particular gene of interest, cell background, and edit. 

                              Explore catalog


                            ​

                            Don't see the cell line you need in our catalog?

                            Register your interest for your desired knock-out cell line and we will be in touch if it's in our pipeline. 

                            Register your interest


                            ​​







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