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DyLight® Fluorochrome conjugated secondary antibodies

Related

  • Secondary antibodies
    • Biotinylated secondaries
      • Secondary antibody selection guide
        • HRP conjugated
            • IHC resources
              • IHC protocol
                • Detect low abundance proteins
                    • Cell imaging
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                        • Advantages of Alexa Fluor® secondaries

                          An alternative to conventional dyes.

                          DyLight® family of dyes

                          DyLight® fluorochromes exhibit high fluorescence intensity, photostability and water solubility and remain fluorescent from pH 4.0 and pH 9.0. Additionally, the water solubility of the DyLight® fluorochromes allows a high dye-to-antibody ratio to be achieved without causing precipitation of conjugates.

                          DyLight® fluorochromes have absorption maxima ranging from 350 nm to 777 nm, covering the entire visible light spectrum. Both the absorption and emission properties of the DyLight® fluorochromes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation.

                          Our catalog includes secondary antibodies conjugated with DyLight® 488, DyLight​® 550, DyLight​® 594 and DyLight® 650.

                          Fig 1. Emission spectra of DyLight®​ fluorochromes available in our catalog. 

                          Antibody conjugate highlights

                          • Large range of different specificities
                          • ​Optimized for conjugate performance - affinity-purified or pre-adsorbed antibodies
                          • Consistent and stable - stable for 1 year at 4°C, providing consistent performance for the duration of use
                            ​

                          Advantages

                          ​

                          • Bright fluorescence - intense emission provides high sensitivity
                          • Narrow emission spectra - negligible bleed-through between fluorochrome channels makes multi-color imaging easy
                          • Highly photostable - better resistance to photobleaching
                          • ​Completely stable in buffers ranging from pH 4.0-9.0
                          • Instrument compatibility - DyLight® can be visualized with common laser and filter settings


                          DyLight® dye properties

                          DyLight® fluorochromes are suitable for fluorescence microscopy and flow cytometry.

                          FluorochromeColorEx/EmSpectraSpectrally equivalent dyes
                          DyLight® 488
                          493/518View graphFITC, Cy2®
                          DyLight® 550
                          562/576View graphCy3®, TRITC
                          DyLight® 594
                          593/618View graphTexas Red®
                          DyLight® 650
                          652/672View graphCy5®

                          Table 1. Spectral properties of DyLight®​ fluorochromes. 

                          ε: Molar extinction coefficient at the absorption maximum

                          For further information or help, contact our Scientific Support team.


                          ​DyLight®​ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

                          Cy®​ and CyDye® are trademarks of Cytiva. 

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