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DyLight® fluorochromes exhibit high fluorescence intensity, photostability and water solubility and remain fluorescent from pH 4.0 and pH 9.0. Additionally, the water solubility of the DyLight® fluorochromes allows a high dye-to-antibody ratio to be achieved without causing precipitation of conjugates.
DyLight® fluorochromes have absorption maxima ranging from 350 nm to 777 nm, covering the entire visible light spectrum. Both the absorption and emission properties of the DyLight® fluorochromes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation.
Our catalog includes secondary antibodies conjugated with DyLight® 488, DyLight® 550, DyLight® 594 and DyLight® 650.
Fig 1. Emission spectra of DyLight® fluorochromes available in our catalog.
DyLight® fluorochromes are suitable for fluorescence microscopy and flow cytometry.
Fluorochrome | Color | Ex/Em | Spectra | ε (M-1cm-1) | Spectrally equivalent dyes |
DyLight® 488 | 493/518 | View graph | 70K | FITC, Cy2® | |
DyLight® 550 | 562/576 | View graph | 150K | Cy3®, TRITC | |
DyLight® 594 | 593/618 | View graph | 80K | Texas Red® | |
DyLight® 650 | 652/672 | View graph | 250K | Cy5® |
Table 1. Spectral properties of DyLight® fluorochromes.
ε: Molar extinction coefficient at the absorption maximum
For further information or help, contact our Scientific Support team.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidaries. Cy® and CyDye® are trademarks of GE Healthcare Limited.