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The use of CRISPR-Cas9 knock-out cell lines to validate antibody specificity, for target identification, or for investigating the relationship between genotype and phenotype is increasing 1. Viewed as the industry gold standard for reagent validation and offering new and previously unavailable insights into the cellular proteome this technology is a powerful part of the molecular biologists' toolkit.
Despite the clear usefulness of CRISPR knock-out cell lines to life science researchers, they do not typically offer the quick solution that you may need. Most researchers take up to five months to generate a CRISPR knock-out cell line, with an average of 3-4 repeats of the CRISPR workflow before they obtain the specific CRISPR edits required 2. This means that incorporating knockout cell lines into your experiments is often not as straightforward as you’d hoped.
Our new range of CRISPR knock-out cell lines are intended to help with this precise problem. Ready-made CRISPR knock-out cell lines that are waiting to be shipped mean that you don't have to spend months generating a knock-out cell line to get the results you need.
We have established a large and expanding range of ready-made knock-outs in commonly used cell lines that are ready-to-go so that obtaining the CRISPR knock-out cell line that you need is as easy as buying a reagent. Our CRISPR knock-out cell line catalog is designed to directly cut more than four months from your preparation time, taking you from five months of experimental work to a simple click and wait for delivery to achieve the CRISPR knock-out cell line you need for your work.
Journals are increasingly calling for research validation using knock-out cell lines to support publication 3. Yet more than 70% of researchers fail to adequately validate their antibodies in their experimental set-up, citing time constraints as the reason 4-6. With such long timelines to generate the necessary CRISPR knock-out cell lines as research tools rather than actual data, it is simple to understand why this is the case.
By reducing your preparation time from the standard five months needed to generate a CRISPR knock-out cell line to the time it takes to order it online, you can reclaim months of experimental time, get more data and publish faster.
One of the common points of failure in the development of CRISPR knock-out cell lines is in confirming the accuracy of the edited cell line. This requires multiple techniques to confirm genetic and proteomic knock-out of the specific gene target. To provide you with the assurance you need and quality you expect from Abcam, our readily available knock-out cell lines are extensively validated using Sanger sequencing, and in many cases, Western blotting, to confirm full gene ablation at the genomic and proteomic levels. All this data is available with each specific CRISPR knock-out cell line.
Using our CRISPR knock-out cell lines offers a consistent and validated cell supply, ensuring the reproducibility of your research, whilst offering you maximal efficiency and removing months of lab-time to get the tools you need to generate data. Additionally, with the use of our Abpromise® guarantee our multilingual support teams are always on hand to help with any questions you may have regarding our CRISPR knock-out cell lines, and offer peace of mind with replacements or refunds available if your CRISPR knock-out cell line does not perform as stated on the datasheet.
With our new catalog of CRISPR knock-out cell lines covering over 1000 key targets in industry-standard cell line backgrounds, such as HeLa and HEK293T, you can quickly and easily reclaim months of lab time and know that you are working with high quality, reproducible research tools to accelerate your research.