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Knock-out cell lines

Related

  • CRISPR KO for antibody validation
    • Knock-out cell lysates
      • Improving efficiency in drug discovery and development

        Knock-out cell lines are powerful tools to understand disease mechanisms and validate potential therapeutic targets

        • Gene editing services
          • Benefits of our gene-edited cell lines
        • Abcam’s strategy for generating high-quality gene-edited cell lines
        • Gene editing resources
          • Introduction to gene editing
          • Gene-edited cell lines in drug discovery
          • Using gene-edited cell lines for validation
          • Webinars
          • Case studies and publications
        • FAQs


        Progressing a drug from target validation to market authorization is a long journey, and CRISPR technology has played a critical role in streamlining this process.

        Knock-out (KO) cell lines help overcome the significant challenge of accessing the correct controls during assay development and enable confident interrogation of the relationship between genotype and phenotype.

        Before creating KO cell lines, there are several factors to consider, and the process can take many months. Using high-quality ready-made cell lines is the quickest and most cost-effective option to accelerate your program.

        Find your KO cell line


        Gene editing services

        ​Gene-edited cell lines play an important role in many stages of the drug discovery pipeline, including validating reagents and targets. We offer an extensive catalog of high-quality ready-made and made-to-order KO cell lines so you can save time and move your project forward.



        Benefits of our gene-edited cell lines:

        • Quickly find your target with our easy-to-navigate catalog and ordering process
        • Physiologically relevant and certified mycoplasm-free cell backgrounds
        • Controls are provided with all orders to help you produce robust, reproducible results


        Abcam's strategy for generating high-quality gene-edited cell lines

        Gene-edited cell lines must undergo rigorous quality control checks to produce consistent and robust data throughout your workflow. 

        We have optimized our CRISPR editing platform to ensure quality at every stage and generate well-characterized cell lines. Our process includes:

        • Comprehensive web-based gRNA design tools for increased accuracy
        • A dual gRNA system resulting in over 95% efficiency
        • Delivering CRISPR ribonucleoprotein complexes directly into cells, so no extra genetic material is left behind
        • Thorough genomic and proteomic validation to confirm the KO, using highly-specific recombinant monoclonal antibodies where possible


        Learn more about how we generate KO cell lines


        Gene editing resources

        Introduction to gene editing

        • Generating cell knock-outs with CRISPR-Cas9 technology: learn more about the CRISPR-Cas9 system in addition to advantages and considerations of this technique
        • Five steps to watch out for when generating KO cell lines: learn how to avoid common pitfalls when generating KO cell lines
        • Fast track your research with KO cell lines: see how using high-quality ready-made KO cell lines can cut months off your timeline
        • Do you need a KO cell line or lysate?: Use our infographic to quickly find out if you need a KO cell line or lysate based on your research question and assay type


        Gene-edited cell lines in drug discovery

        • How you can use KO cell lines: explore the many applications for KO cell lines, including drug discovery, assay development, reagent validation, and pathway investigation
        • Using KO cell lines in assay development: our experienced cell lines team discusses how KO cell lines can streamline assay development
        • How CRISPR cell engineering accelerates the drug pipeline: we highlight how CRISPR cell engineering can cut pipeline attrition at several stages, including target identification and lead optimization


        Using gene-edited cell lines for validation

        • KO validation: confirming antibody specificity: see how we use KO validation to improve antibody specificity in our catalog
        • KO validation: overcoming the perils of poor antibody specificity: discover how KO cell lines can improve antibody validation and increase reproducibility.


        Webinars

        • Knock-out cell lines and their role in confirming antibody specificity: Listen to Dr Will Howatt, Abcam’s Director of Antibody Validation, as he describes the role KO cell lines have in antibody validation
        • Understanding physiologically relevant pathways using Abcam’s knock-out cell lines: Abcam application scientists Frank He and Daniel Wing outline Abcam’s cell engineering capabilities and how KO cell lines can be used to investigate signaling pathways
        • Harnessing the power of knock-out validation for research reproducibility: Dr Hanna Dreja, Abcam’s Principal Quality Control Scientist, discusses how KO cell lines can increase reproducibility across the life science industry


        Case studies and publications

        • Applying CRISPR to the drug discovery pipeline: read case studies on how CRISPR cell lines are being used to accelerate the drug discovery pipeline
        • CRISPR KO cell line publications: a collection of publications demonstrating the use of KO cell lines
        • KO cell lines and lysates for COVID-19 research: a curated list of high-quality products to help accelerate your COVID-19 research



        Frequently asked questions (FAQs)

        Get the answers to frequently asked questions about our knockout cell lines: 

        • How are your knock-out cell lines generated?
        • Do you test your KO cell lines for mycoplasma? Can I obtain a copy of the mycoplasma-free certificate?
        • How should I store the cell when I receive them?
        • Can I use my own WT cell line, or do I have to purchase it from you? Which wild type (WT) cell line should I purchase to use as a control with my KO cell line?
        • What is the biological safety level of your cells?
        • What guarantees are in place for your knock-out cell lines?
        • What is the passage number of the cell lines?
        • Are there antibiotics in the cells?
        • How should I culture my cells?
        • What is the standard media formualtion your cells are in?
        How are your knock-out (KO) cell lines generated?

        Our KO cell lines were generated using CRISPR/Cas9 technology. The KO cell lines are single-clonal and validated using Sanger sequencing. Please refer to the datasheet for the specific mutation description.  

        Do you test the KO cell lines for mycoplasma?

        Yes, the cell line banks are tested for mycoplasma using an independent third party, Bionique, using broth and agar method. We also spot-check our stocks regularly. 

        Can I obtain a copy of the mycoplasma-free certificate?

        Yes. Please contact our scientific support team and they can provide this to you.

        How should I store the cells once I receive them?

        If you do not plan to resuscitate immediately, we recommend that you store the vial in liquid nitrogen immediately upon receipt.

        Can I use my own WT cell line, or do I have to purchase it from you?

        We recommend you use our WT, which is provided free of charge with each knock-out cell line order (not available for individual purchase). Please refer to the datasheet to determine which WT to add to your KO cell line order. Alternatively, you can contact us if you have any questions. 

        Which wild type (WT) cell line should I purchase to use as a control with my KO cell line?

        When you buy a KO cell line the WT is available free of charge, however you will need to add it separately to your order. Please refer to the General Notes section within the KO cell line's datasheet for our recommended WT and how to order. If you have questions you can contact our scientific support team.

        What is the biological safety level (BSL) of your cells?

        Our KO cell lines are classified as BSL1 and BSL2 depending on the cell line. Cell lines such as A549, HCT116, HEPG2, and MCF7 are BSL1. EK-293T and HeLa cell lines are BSL2. 

        What guarantees are in place for your KO cell lines?

        Our KO cell lines are covered by our Abpromise™ guarantee. This means we guarantee the cell lines are mycoplasma free and validated at the genomic level by Sanger sequencing. 

        What is the passage number of the cell lines?

        The cells are all less than passage number 20. Please contact us if you would like the exact passage number of your lot.

        Are there antibiotics in the cells?

        The clones are selected using puromycin resistance, however the cells are not grown in the presence of puromycin or other antibiotics.

        How should I culture my cells?

        Please refer to the handling procedure on the KO cell line's datasheet and our cell culture protocol. 

        What is the standard media formulation that your cells are in?

        The cells are in Cell Freezing Medium-DMSO serum-free media. Please refer to the KO cell line's datasheet for more information.






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