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Our KO catalog range and custom service makes it easier to get the gene edited cell lines you need
Our reliable, expertly engineered cell lines allow you to confidently interrogate the relationship between genotype and phenotype without having to establish your own gene edited cell line.
While CRISPR can be beneficial for antibody validation, the process can be tedious and involves numerous, complex steps. One small slip could result in weeks of wasted time and money that may not be evident until the end.
Why not explore our ready-made KO cell lines catalog?
|We can also develop and supply the right custom gene-edited cell line to meet your needs.|
Whether you need knockouts or knock-ins, our CRISPR cell line specialists will deliver the custom engineered cells you need with the hassle.
Register your interest for your desired knock-out cell line and we will be in touch if it's in our pipeline.
Use our convenient infographic to quickly find out based on your research question and assay type.
Our KO cell lines were generated using CRISPR/Cas9 technology. The KO cell lines are single-clonal and validated using Sanger sequencing. Please refer to the datasheet for the specific mutation description.
Yes, the cell line banks are tested for mycoplasma using an independent third party, Bionique, using broth and agar method. We also spot-check our stocks regularly.
Yes. Please contact our scientific support team and they can provide this to you.
If you do not plan to resuscitate immediately, we recommend that you store the vial in liquid nitrogen immediately upon receipt.
You can purchase the appropriate WT from us or receive it free of charge if ordered with a KO cell line, as these have been made by Abcam’s standard procedures. Please refer to the datasheet to determine which WT to order with your KO cell line. Alternatively, you can contact us if you have any questions.
When you buy a KO cell line the WT is available free of charge, however you will need to add it separately to your order. Please refer to the General Notes section within the KO cell line's datasheet for our recommended WT and how to order. If you have questions you can contact our scientific support team.
Our KO cell lines are classified as BSL1 and BSL2 depending on the cell line. Cell lines such as A549, HCT116, HEPG2, and MCF7 are BSL1. EK-293T and HeLa cell lines are BSL2.
Our KO cell lines are covered by our Abpromise® guarantee. This means we guarantee the cell lines are mycoplasma free and validated at the genomic level by Sanger sequencing.
The cells are all less than passage number 20. Please contact us if you would like the exact passage number of your lot.
The clones are selected using puromycin resistance, however the cells are not grown in the presence of puromycin or other antibiotics.
Please refer to the handling procedure on the KO cell line's datasheet and our cell culture protocol.
The cells are in Cell Freezing Medium-DMSO serum-free media. Please refer to the KO cell line's datasheet for more information.