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Progressing a drug from target validation to market authorization is a long journey, and CRISPR technology has played a critical role in streamlining this process.
Knock-out (KO) cell lines help overcome the significant challenge of accessing the correct controls during assay development and enable confident interrogation of the relationship between genotype and phenotype.
Before creating KO cell lines, there are several factors to consider, and the process can take many months. Using high-quality ready-made cell lines is the quickest and most cost-effective option to accelerate your program.
Gene-edited cell lines play an important role in many stages of the drug discovery pipeline, including validating reagents and targets. We offer an extensive catalog of high-quality ready-made KO cell lines so you can save time and move your project forward. If you require a custom line, partner with our experienced cell engineering team to create the line you need.
Gene-edited cell lines must undergo rigorous quality control checks to produce consistent and robust data throughout your workflow.
We have optimized our CRISPR editing platform to ensure quality at every stage and generate well-characterized cell lines. Our process includes:
Our KO cell lines were generated using CRISPR/Cas9 technology. The KO cell lines are single-clonal and validated using Sanger sequencing. Please refer to the datasheet for the specific mutation description.
Yes, the cell line banks are tested for mycoplasma using an independent third party, Bionique, using broth and agar method. We also spot-check our stocks regularly.
Yes. Please contact our scientific support team and they can provide this to you.
If you do not plan to resuscitate immediately, we recommend that you store the vial in liquid nitrogen immediately upon receipt.
We recommend you use our WT, which is provided free of charge with each knock-out cell line order (not available for individual purchase). Please refer to the datasheet to determine which WT to add to your KO cell line order. Alternatively, you can contact us if you have any questions.
When you buy a KO cell line the WT is available free of charge, however you will need to add it separately to your order. Please refer to the General Notes section within the KO cell line's datasheet for our recommended WT and how to order. If you have questions you can contact our scientific support team.
Our KO cell lines are classified as BSL1 and BSL2 depending on the cell line. Cell lines such as A549, HCT116, HEPG2, and MCF7 are BSL1. EK-293T and HeLa cell lines are BSL2.
Our KO cell lines are covered by our Abpromise® guarantee. This means we guarantee the cell lines are mycoplasma free and validated at the genomic level by Sanger sequencing.
The cells are all less than passage number 20. Please contact us if you would like the exact passage number of your lot.
The clones are selected using puromycin resistance, however the cells are not grown in the presence of puromycin or other antibiotics.
Please refer to the handling procedure on the KO cell line's datasheet and our cell culture protocol.
The cells are in Cell Freezing Medium-DMSO serum-free media. Please refer to the KO cell line's datasheet for more information.