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CRISPR/Cas9 genome editing technology is a powerful tool that has allowed us to develop an extensive range of knock-out cell lysates.
Our range provides you with:
Each KO cell line is individually cloned and validated by Sanger sequencing. The KO cell lysates are provided with the parental wild-type lysate to allow the biological impact of the knockout to be assessed within a consistent cellular background.
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Use our convenient infographic to quickly find out based on your research question and assay type.
Get the answers to frequently asked questions about our knock-out cell lysates range:
In July 2019, Abcam acquired the entire portfolio of diploid KO cell lines from Edigene. The KO cell lines are single clonal, generated using CRISPR/Cas9 technology, and validated using sanger sequencing. Please refer to the product’s datasheets for the specific mutation information. For details of how the lysates were prepared, please refer to our lysis protocol.
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ration 300:100:10). Please refer to our lysis protocol for further details on how the lysates were prepared.
RIPA buffer: 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.
Please refer to the product’s datasheet for reconstitution instructions.
Once reconstituted, we recommend you store the lysate at -20 degrees for short-term storage or -80 degrees for long-term storage. For further details please refer to the product’s datasheet.
You will receive 100µg of the lyophilized KO lysate & 100µg of the appropriate lyophilized wild-type/parental cell line. You can find full details on the product’s datasheet.
Please only use the wild-type lysate provided when you purchased the KO lysate.
The lysates are denatured.
We use the extensive library of human, KO haploid cell lines generated via CRISPR/Cas9 for our antibody validation program, and we will be adding to this using our new range of diploid KO cell lines.
Unfortunately, we are not able to provide the gRNA sequence.