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Mounting media

Related

  • IHC guide: introduction
    • Tips for experimental design
        • IHC guide: sample prep
          • Tissue fixation, embedding and sectioning
            • Antigen retrieval
              • Permeabilization
                • Blocking
                    • IHC guide: immunostaining
                      • Choosing and optimizing primary antibodies
                        • Direct vs indirect detection
                          • Secondary antibodies for IHC
                            • Chomogenic detection
                              • Substrates and chromogens
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                                  • Counterstaining and special stains
                                    • Mounting
                                        • IHC guide: troubleshooting
                                            • IHC guide: controls

                                              Mounting a tissue specimen is essential for preserving the specimen during storage as well as for enhancing imaging quality during microscopy.

                                              After immunochemical staining, mounting media are used to adhere a coverslip to a tissue section or cell smear. Mounting media are important to protect the specimen and staining from physical damage.  Mounting media also play an important role in improving the clarity and contrast of an image during microscopy.​

                                              There are two categories of mounting media, organic and aqueous (or hydrophobic and hydrophilic, respectively). Organic mounting media can only be used for enzymatic labels where the precipitate formed between the enzyme and the chromogen is not soluble in the organic solvents used during mounting of the tissue (eg diaminobenzidine, DAB).

                                              Aqueous mounting media are generally suitable for all enzymatic label/chromogen combinations and fluorescent labels.

                                              ​

                                              Recommended mounting media for non-fluorescent imaging


                                              Product nameTypeProduct code
                                              Limonene Mounting MediumOrganicab104141
                                              Mounting Medium for IHCAqueousab64230


                                              Recommended mounting media for fluorescent imaging

                                              For fluorescent imaging, mounting media that preserve fluorescence in tissue and cytological smears, are used, enabling imaging of samples after prolonged storage.

                                              Product nameTypeProduct code
                                              Anti-Fade Mounting MediumAqueousab104135
                                              Anti-Fade Mounting Medium With DAPIAqueousab104139
                                              Anti-Fade Mounting Medium With PIAqueousab104129

                                              >> Next page: troubleshooting IHC experiments

                                              << Previous page: counterstaining

                                              ​Download the full IHC guide

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