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Investigate p21 and its effectors with highly-cited tools.
We have put together the list of high impact products for your studies on the cyclin-dependent kinase (CDK) inhibitor p21 (also known as WAF1, CAP20, Cip1, and Sdi1). Browse the most-cited research targets and well-referenced products for better understanding p21 in cellular function.
The p21 toolkit
|Type: Rabbit monoclonal antibody|
Applications: ICC/IF, Flow Cyt, WB, IP, IHC-P
|Anti-p21 antibody||Biotin conjugated||ab79467|
|Goat Anti-Rabbit IgG H&L||Alexa Fluor® 488 conjugated||ab150077|
|IRDye® 800CW (preadsorbed)||ab216773|
p21 SimpleStep ELISA® kit
|Quantitatively measure p21 in human cell and tissue extracts. Get fast, reproducible results in 90 minutes.|
Range: 62.5 pg/ml - 4000 pg/ml
|Protein||Recombinant p21 protein||Human p21 protein for use in WB and as control1.||ab56278|
|Small molecule compound||UC2288 (inhibitor)|
|Selective p21 inhibitor structurally related to Sorafenib (ab141966). Inhibits cytosolic p21 (IC50 = 10 µM) and functions independently of p53 2 3.||ab146969|
|Tenovin-1 (activator)||p21 activator and inducer of cell cycle arrest4.||ab120889|
Activation of p53-p21 pathway
DNA damaging agents activate the p53-p21 pathway causing cell cycle arrest and DNA repair5. This mechanism is exploited by chemotherapeutic agents that induce p21 as a means of inhibiting cancer progression. Investigating p21 regulation in a p53-dependent or -independent manner can form a key basis of tumor suppression6, 7.
|Oxaliplatin||Inducer, inhibitor||Exhibits dual role in p53-dependent and -independent pathways. Causes G0-G1 arrest in p53 wild type cells and p21 degradation in p53 mutants8.||ab141054|
|Nocodazole||Inducer||p21 inducer that increases expression of p21 in a p53-dependent fashion in absence of DNA damage9, 10.||ab120630|
|Cycloheximide (CHX)||Modulator||Induction of p21 during serum stimulation (indicating serum contains a p21 blocking element)7. CHX traditionally acts as a p21 inhibitor promoting apoptosis in serum-starved cells11.||ab120093|
|PMA||Inducer||p53-independent p21 induction via Sp1 binding sites within p21 promoter12.||ab120297|
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