Protease and phosphatase inhibitor cocktails
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Protect your protein samples from degradation and preserve their phosphorylation state with our convenient and easy to use protease and phosphatase inhibitor cocktails.
You can use different inhibitor combinations for your experiment, as well as water soluble & DMSO versions. Browse the guide below for more information.
Protease inhibitor cocktails
Cell lysates contain endogenous proteases which degrade proteins and can affect sensitivity and reliability in protein expression research. It is therefore common practice to add protease inhibitors to lysate samples to protect proteins of interest. Our protease inhibitor cocktails inhibit a number of proteases including serine, cysteine and aspartic proteases.
| Kit name | Key information | Inhibitors included | Target protease/phosphatase |
| Optimized to generate the maximal protection to mammalian tissue and cell extracts. | Aprotinin, Leupeptin, Pepstain A, PMSF | Acidic protease, Cysteine protease, Mammalian acetylcholinesterase, Serine protease | |
| Protease Inhibitor Cocktail (EDTA-free) | Ideal for mamalian cell/tissue lysates and bacterial cell extracts using metal chelation chromatography | AEBSF hydrochloride, Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, Pepstatin A | Aminopeptidase B and leucine aminopeptidase, Aspartic protease, Cysteine protease, Serine protease |
| Protease Inhibitor Cocktail II | General use cocktail | AEBSF hydrochloride, Aprotinin (bovine lung), E-64, EDTA disodium salt, Leupeptin hemisulfate | Cysteine protease, Metalloprotease, Serine protease |
| Protease Inhibitor Cocktail III | Ideal for bacterial cell extracts using metal chelation chromatography | AEBSF hydrochloride, Bestatin, E-64, EDTA disodium salt, Pepstatin A, | Aminopeptidase B and leucine aminopeptidase, Aspartic protease, Cysteine protease, Metalloprotease, Serine protease |
| Protease Inhibitor Cocktail IV | Ideal for fungal and yeast cell extracts | AEBSF hydrochloride, E-64, Pepstatin A, o-Phenanthroline | Aspartic protease, Cysteine protease, Metalloprotease, Serine protease |
Phosphatase inhibitor cocktails
Approximately 30% of known proteins can be reversibly phosphorylated in order to control a number of cellular processes. Protein kinases predominantly phosphorylate at serine, threonine and tyrosine residues - phosphatases reverse this phosphorylation. When studying these processes and pathways it is therefore essential to preserve the phosphorylation state of these proteins in your samples. Our phosphatase inhibitor cocktails inhibit a number of phosphatases including specific serine/threonine and tyrosine phosphatases as well as non-specific acid and alkaline phosphatases.
| Kit name | Inhibitors included | Target protease/phosphatase |
| Phosphatase Inhibitor Cocktail I | β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate | Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase |
| Phosphatase Inhibitor Cocktail II | Imidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrate | Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase |
| Phosphatase Inhibitor Cocktail III | (-)-p-Bromotetramisole oxalate, Cantharidin, Calyculin A | Alkaline Phosphatase, Serine/threonine Phosphatase (PP1 and PP2A) |
| Phosphatase Inhibitor Cocktail IV | β-Glycerophosphate, Imidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrate | Acid Phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase |
Protease and phosphatase inhibitor cocktails
Combined protease and phosphatase inhibitor cocktail. Ideal for the ultimate protection of your protein samples. Available with and without EDTA, a metalloproteinase inhibitor.
| Kit name | Key information | Inhibitors included | Target protease/phosphatase |
| Protease and Phosphatase Inhibitor Cocktail | AEBSF-free and mass spectroscopy compatible - water soluble | Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate, EDTA disodium salt | Aminopeptidase B and leucine aminopeptidase, Cysteine protease, Metalloprotease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase |
| Protease and Phosphatase Inhibitor Cocktail (EDTA-free) | AEBSF-free and mass spectroscopy compatible - water soluble | Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate | Aminopeptidase B and leucine aminopeptidase, Cysteine protease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase |