Overview

  • Product name

  • Description

    Rabbit polyclonal to REC8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human REC8 aa 147-225.
    Sequence:

    PSPFDIPQIRHLLEAAIPERVEEIPPEVPTEPREPERIPVTVLPPEAITI LEAEPIRMLEIEGERELPEVSRRELDLLI


    Database link: O95072

  • Positive control

    • WB: RT4 whole cell lysate. IHC-P: Human testis tissue. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab246985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.4 µg/ml. Predicted molecular weight: 63 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function

    Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
  • Tissue specificity

    Expressed in testis and thymus.
  • Sequence similarities

    Belongs to the rad21 family.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Nucleus. Chromosome. In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cohesin Rec8p antibody
    • Human homolog of rad21 S. pombe antibody
    • Meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family antibody
    • Meiotic recombination protein REC8 homolog antibody
    • Meiotic recombination protein REC8-like 1 antibody
    • rec8 antibody
    • REC8 homolog (yeast) antibody
    • REC8 like 1 antibody
    • REC8_HUMAN antibody
    • Rec8p antibody
    • Recombination and sister chromatid cohesion protein homolog antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for REC8 (green) using ab246985 at 4 μg/ml in ICC/IF.

  • Paraffin-embedded human testis tissue stained for REC8 using ab246985 at 1/500 dilution in immunohistochemical analysis.
  • Anti-REC8 antibody (ab246985) at 0.4 µg/ml + RT4 (human urinary bladder cancer cell line) whole cell lysate

    Predicted band size: 63 kDa

References

ab246985 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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