Anti-RECK antibody (ab115844)

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Overview

  • Product name

    Anti-RECK antibody
    See all RECK primary antibodies

  • Description

    Rabbit polyclonal to RECK

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human

  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 580-629 (HGTSFTIDCN VCSCFAGNLV CSTRLCLSEH SSDDDRRTFT) of Mouse RECK (NP_057887).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Mouse spleen lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 106 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.

  • Tissue specificity

    Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.

  • Sequence similarities

    Contains 3 Kazal-like domains.

  • Post-translational
    modifications

    N-glycosylated.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • hRECK antibody
    • Membrane anchored glycoprotein (metastasis and invasion) antibody
    • RECK antibody
    • RECK protein antibody
    • RECK_HUMAN antibody
    • Reversion inducing cysteine rich protein with Kazal motifs antibody
    • Reversion-inducing cysteine-rich protein with Kazal motifs antibody
    • ST15 antibody
    • Suppression of tumorigenicity 15 (reversion inducing cysteine rich protein with kazal motifs) antibody
    • Suppressor of tumorigenicity 15 antibody
    • Suppressor of tumorigenicity 15 protein antibody
    see all

Images

  • Western blot - Anti-RECK antibody (ab115844)
    Western blot - Anti-RECK antibody (ab115844)
    Anti-RECK antibody (ab115844) at 1 µg/ml + Mouse spleen lysate at 10 µg

    Predicted band size: 106 kDa



    Gel concentration: 6-18%

References

This product has been referenced in:

  • Mao Y  et al. Alkannin restrains oral squamous carcinoma cell growth, migration and invasion by regulating microRNA-9/RECK axis. Artif Cells Nanomed Biotechnol 47:3153-3162 (2019). Read more (PubMed: 31349748) »
  • Jian Y  et al. Down-regulated microRNA-30b-3p inhibits proliferation, invasion and migration of glioma cells via inactivation of the AKT signaling pathway by up-regulating RECK. Biosci Rep 39:N/A (2019). Read more (PubMed: 31270250) »
See all 3 Publications for this product

Publishing research using ab115844? Please let us know so that we can cite the reference in this datasheet.

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