Anti-RECK antibody [MM0523-9G19] (ab89915)
![Western blot - Anti-RECK antibody [MM0523-9G19] (ab89915)](https://www.abcam.com/ps/products/89/ab89915/Images/ab89915-256871-anti-reck-antibody-mm0523-9g19-western-blot.jpg "Western blot - Anti-RECK antibody [MM0523-9G19] (ab89915)")
Key features and details
- Mouse monoclonal [MM0523-9G19] to RECK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-RECK antibody [MM0523-9G19]
See all RECK primary antibodies -
Description
Mouse monoclonal [MM0523-9G19] to RECK -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length Human RECK protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by protein G affinity chromatography and 0.2 µm filtered. -
Clonality
Monoclonal -
Clone number
MM0523-9G19 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 106 kDa.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 106 kDa. |
Target
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Function
Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression. -
Tissue specificity
Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells. -
Sequence similarities
Contains 3 Kazal-like domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 313488 Human
- Entrez Gene: 8434 Human
- Omim: 605227 Human
- SwissProt: O95980 Human
- SwissProt: Q5W0K6 Human
- Unigene: 388918 Human
- Unigene: 728961 Human
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Alternative names
- hRECK antibody
- Membrane anchored glycoprotein (metastasis and invasion) antibody
- RECK antibody
see all
Images
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Western blot - Anti-RECK antibody [MM0523-9G19] (ab89915)Anti-RECK antibody [MM0523-9G19] (ab89915) at 1/500 dilution + Human liver lysate
Predicted band size: 106 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab89915 has not yet been referenced specifically in any publications.