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what mass ofGFP protein is being measured in the fluorescence graph, where GFP emmission is measured against uM GFP?
Asked on Sep 26 2012
The molecular weight of GFP is 27 kDa. A 1.0 uM solution of GFP contains 27 micrograms of GFP per milliliter of solution. In this assay, 200 ul is added to each well, so for the 1 uM standard, the well contains 5.4 ug, and for the 2 uM standard, 10.8 ug, and so on.
Answered on Sep 26 2012