Recombinant Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134)
![Western blot - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134)](https://www.abcam.com/ps/products/134/ab134134/Images/ab134134-148378-anti-abl2-antibody-epr12222-western-blot.jpg "Western blot - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1222(2)] to ABL1 + ABL2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] -
Description
Rabbit monoclonal [EPR1222(2)] to ABL1 + ABL2 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 85% identity with ABL1. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ABL2 aa 500-600. The exact sequence is proprietary.
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Positive control
- Jurkat cell lysates; HeLa cell lysates; K562 cell lysates; Molt 4 cell lysates; RAW 264.7 cell lysates; PC 12 cell lysates; NIH 3T3 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.36 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1222(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 128 kDa.
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| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 128 kDa. |
Target
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Cellular localization
ABL1: Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear. ABL2: Cytoplasm, cytoskeleton. -
Database links
- Entrez Gene: 25 Human
- Entrez Gene: 27 Human
- Entrez Gene: 11350 Mouse
- Entrez Gene: 11352 Mouse
- Entrez Gene: 304883 Rat
- Entrez Gene: 311860 Rat
- Omim: 164690 Human
- Omim: 189980 Human
see all
Images
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Western blot - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134)All lanes : Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MOLT 4 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 128 kDa -
![Flow Cytometry (Intracellular) - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134)](https://www.abcam.com/ps/products/134/ab134134/Images/ab134134-148377-anti-abl2-antibody-epr12222-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134)Intracellular flow cytometric analysis of permeabilized Jurkat cells labelling ABL2 (red) using ab134134 at 1/10 dilution or using a Rabbit IgG negative control (green)
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![OI-RD Scanning - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134)](https://www.abcam.com/ps/products/134/ab134134/Images/ab134134-148376-anti-abl2-antibody-epr12222.jpg)
OI-RD Scanning - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134) -
![Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134)](https://www.abcam.com/ps/products/134/ab134134/Images/ab134134-4-benefits-of-recombinant-antibodies.png)
Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab134134 has been referenced in 2 publications.
- Klein LE et al. A humanin analog decreases oxidative stress and preserves mitochondrial integrity in cardiac myoblasts. Biochem Biophys Res Commun 440:197-203 (2013). WB . PubMed: 23985350
- Hutterer C et al. Profiling of the kinome of cytomegalovirus-infected cells reveals the functional importance of host kinases Aurora A, ABL and AMPK. Antiviral Res 99:139-48 (2013). WB ; Human . PubMed: 23648710