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    recombinant-abl1--abl2-antibody-epr12222-bsa-and-azide-free-ab248694.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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RecombinantRabMAb

Recombinant Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)

  • Datasheet
  • Certificate of Compliance
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Western blot - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)
  • Flow Cytometry (Intracellular) - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)
  • OI-RD Scanning - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)
  • Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1222(2)] to ABL1 + ABL2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

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Overview

  • Product name

    Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free
  • Description

    Rabbit monoclonal [EPR1222(2)] to ABL1 + ABL2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248694 is the carrier-free version of ab134134.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 2.36 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1222(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • HeLa whole cell lysate (ab29545)
    • RAW 264.7 whole cell lysate (ab7187)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant human ABL2 protein (ab51259)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 128 kDa.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 128 kDa.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Cellular localization

    ABL1: Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear. ABL2: Cytoplasm, cytoskeleton.
  • Database links

    • Entrez Gene: 25 Human
    • Entrez Gene: 27 Human
    • Entrez Gene: 11350 Mouse
    • Entrez Gene: 11352 Mouse
    • Entrez Gene: 304883 Rat
    • Entrez Gene: 311860 Rat
    • Omim: 164690 Human
    • Omim: 189980 Human
    • SwissProt: P00519 Human
    • SwissProt: P42684 Human
    • SwissProt: P00520 Mouse
    • SwissProt: Q4JIM5 Mouse
    • Unigene: 159472 Human
    • Unigene: 431048 Human
    • Unigene: 1318 Mouse
    • Unigene: 329515 Mouse
    • Unigene: 474779 Mouse
    see all

Images

  • Western blot - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)
    Western blot - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)
    All lanes : Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] (ab134134) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : MOLT 4 cell lysate
    Lane 5 : RAW 264.7 cell lysate
    Lane 6 : PC12 cell lysate
    Lane 7 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 128 kDa



    This data was developed using ab134134, the same antibody clone in a different buffer formulation.

  • Flow Cytometry (Intracellular) - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)
    Flow Cytometry (Intracellular) - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)

    This data was developed using ab134134, the same antibody clone in a different buffer formulation. 

    Intracellular flow cytometric analysis of permeabilized Jurkat cells labelling ABL2 (red) using ab134134 at 1/10 dilution or using a Rabbit IgG negative control (green)

     

  • OI-RD Scanning - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)
    OI-RD Scanning - Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)
    This data was developed using ab134134, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)
    Recombinant Anti-ABL1 + ABL2 antibody [EPR1222(2)] - BSA and Azide free (ab248694)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab248694? Please let us know so that we can cite the reference in this datasheet.

ab248694 has not yet been referenced specifically in any publications.

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