Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Tags: His tag C-Terminus
- Suitable for: WB, SDS-PAGE, ELISA
Product nameRecombinant AMARV GP protein (His tag)
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceMKTTCLLISLILIQGVKTLPILEIASNIQPQNVDSVCSGTLQKTEDVHLM GFTLSGQKVADSPLEASKRW AFRAGVPPKNVEYTEGEEAKTCYNISVT DPSGKSLLLDPPTNIRDYPKCKTIHHIQGQNPHAQGIALHLW GAFFLY DRIASTTMYRGKVFTEGNIAAMIVNKTVHKMIFSRQGQGYRHMNLTSTNK YWTSSNGTQTNDTG CFGTLQEYNSTKNQTCAPSKKPLPLPTAHPEVKL TSTSTDATKLNTTDPNSDDEDLTTSGSGSGEQEPYT TSDAATKQGLSS TMPPTPSPQPSTPQQGGNNTNHSQGVVTEPGKTNTTAQPSMPPHNTTTIS TNNTSKHN LSTPSVPIQNATNYNTQSTAPENEQTSAPSKTTLLPTENP TTAKSTNSTKSPTTTVPNTTNKYSTSPSPT PDSTAQHLVYFRRKRNIL WREGDMFPFLDGLINAPIDFDPVPNTKTIFDESSSSGASAEEDQHASPNI SL TLSYFPKVNENTAHSGENENDCDAELRIWSVQEDDLAAGLSWIPFF GPGIEGLYTAGLIKNQNNLVCRLR RLANQTAKSLELLLRVTTEERTFS LINRHAIDFLLARWGGTCKVLGPDCCIGIEDLSRNISEQIDQIKKD EQ KEGTGWGLGGKWWTSDWGVLTNLGILLLLSIAVLIALSCICRIFTKYIG
Predicted molecular weight60 kDa including tags
TagsHis tag C-Terminus
Additional sequence informationAngola marburgvirus glycoprotein minus the transmembrane domain (MARV-Angola rGPdTM). The theoretical molecular weight of the protein is ~60 kDa including the His-tag, without glycosylation.
Our Abpromise guarantee covers the use of ab190126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
PBS is supplemented with 10% glycerol, arginine and glutamic acid.
- Envelope glycoprotein
RelevanceGP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. Binding to CD209 and CLEC4M, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses, facilitate infection of macrophages and endothelial cells. These interactions not only facilitate virus cell entry, but also allow capture of viral particles by DCs and subsequent transmission to susceptible cells without DCs infection (trans infection). GP2 acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in GP2, releasing the fusion hydrophobic peptide
Cellular localizationGP2: Virion membrane; Single-pass type I membrane protein. Virion membrane; Lipid-anchor. Host cell membrane; Single-pass type I membrane protein . Host cell membrane; Lipid-anchor By similarity. Note: In the cell, localizes to the plasma membrane lipid rafts, which probably represent the assembly and budding site. GP1: Virion membrane; Peripheral membrane protein. Host cell membrane; Peripheral membrane protein. Note: GP1 is not anchored to the viral envelope, but associates with the extravirion surface through its binding to GP2. In the cell, both GP1 and GP2 localize to the plasma membrane lipid rafts, which probably represent the assembly and budding site.
All lanes : rabbit polyclonal antibody anti AMARV GP at 0.5 µg/ml
Lane 1 :
Recombinant AMARV GP protein (His tag) (ab190126) at 0.1 µg
Lane 2 :
Recombinant AMARV GP protein (His tag) (ab190126) at 0.5 µg
Lane 3 :
Recombinant AMARV GP protein (His tag) (ab190126) at 1 µg
All lanes : anti-rabbit IgG-HRP conjugate, followed by substrate.
Predicted band size: 74 kDa
SDS-PAGE analysis of 1 μg and 5 μg (lanes 1, 2 respectively) of ab190126 under denaturing and reducing conditions.
Plate was coated with ab190126 starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using one dilution of a positive control serum, followed with anti-IgG HRP conjugate and TM substrate. OD650 is reported. Background of ab190126 coated plate without positive control serum was 0.051 OD650.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab190126 has not yet been referenced specifically in any publications.