Recombinant B. pertussis Adenylate Cyclase protein (ab179866)

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Description

  • Product name

    Recombinant B. pertussis Adenylate Cyclase protein

  • Biological activity

    Addition of 10~100 ng/ml of ab179866 to medium significantly increased the levels of cellular cAMP levels in CHO and human monocyte cells.

  • Purity

    > 80 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P0DKX7

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Bordetella pertussis

    • Sequence

      MQQSHQAGYANAADRESGIPAAVLDGIKAVAKEKNATLMFRLVNPHSTSL IAEGVATKGLGVHAKSSDWGLQAGYIPVNPNLSKLFGRAPEVIARADNDV NSSLAHGHTAVDLTLSKERLDYLRQAGLVTGMADGVVASNHAGYEQFEFR VKETSDGRYAVQYRRKGGDDFEAVKVIGNAAGIPLTADIDMFAIMPHLSN FRDSARSSVTSGDSVTDYLARTRRAASEATGGLDRERIDLLWKIARAGAR SAVGTEARRQFRYDGDMNIGVITDFELEVRNALNRRAHAVGAQDVVQHGT EQNNPFPEADEKIFVVSATGESQMLTRGQLKEYIGQQRGEGYVFYENRAY GVAGKSLFDDGLGAAPGVPSGRSKFSPDVLETVPASPGLRRPSLGAVERQ DSGYDSLDGVGSRSFSLGEVSDMAAVEAAELEMTRQVLHAGARQDDAEPG VSGASAHWGQRALQGAQAVAAAQRLVHAIALMTQFGRAGSTNTPQEAASL SAAVFGLGEASSAVAETVSGFFRGSSRWAGGFGVAGGAMALGGGIAAAVG AGMSLTDDAPAGQKAAAGAEIALQLTGGTVELASSIALALAAARGVTSGL QVAGASAGAAAGALAAALSPMEIYGLVQQSHYADQLDKLAQESSAYGYEG DALLAQLYRDKTAAEGAVAGVSAVLSTVGAAVSIAAAASVVGAPVAVVTS LLTGALNGILRGVQQPIIEKLANDYARKIDELGGPQAYFEKNLQARHEQL ANSDGLRKMLADLQAGWNASSVIGVQTTEISKSALELAAITGNADNLKSV DVFVDRFVQGERVAGQPVVLDVAAGGIDIASRKGERPALTFITPLAAPGE EQRRRTKTGKSEFTTFVEIVGKQDRWRIRDGAADTTIDLAKVVSQLVDAN GVLKHSIKLDVIGGDGDDVVLANASRIHYDGGAGTNTVSYAALGRQDSIT VSADGERFNVRKQLNNANVYREGVATQTTAYGKRTENVQYRHVELARVGQ LVEVDTLEHVQHIIGGAGNDSITGNAHDNFLAGGSGDDRLDGGAGNDTLV GGEGQNTVIGGAGDDVFLQDLGVWSNQLDGGAGVDTVKYNVHQPSEERLE RMGDTGIHADLQKGTVEKWPALNLFSVDHVKNIENLHGSRLNDRIAGDDQ DNELWGHDGNDTIRGRGGDDILRGGLGLDTLYGEDGNDIFLQDDETVSDD IDGGAGLDTVDYSAMIHPGRIVAPHEYGFGIEADLSREWVRKASALGVDY YDNVRNVENVIGTSMKDVLIGDAQANTLMGQGGDDTVRGGDGDDLLFGGD GNDMLYGDAGNDTLYGGLGDDTLEGGAGNDWFGQTQAREHDVLRGGDGVD TVDYSQTGAHAGIAAGRIGLGILADLGAGRVDKLGEAGSSAYDTVSGIEN VVGTELADRITGDAQANVLRGAGGADVLAGGEGDDVLLGGDGDDQLSGDA GRDRLYGEAGDDWFFQDAANAGNLLDGGDGRDTVDFSGPGRGLDAGAKGV FLSLGKGFASLMDEPETSNVLRNIENAVGSARDDVLIGDAGANVLNGLAG NDVLSGGAGDDVLLGDEGSDLLSGDAGNDDLFGGQGDDTYLFGVGYGHDT IYESGGGHDTIRINAGADQLWFARQGNDLEIRILGTDDALTVHDWYRDAD HRVEIIHAANQAVDQAGIEKLVEAMAQYPDPGAAAAAPPAARVPDTLMQS LAVNWR

    • Predicted molecular weight

      178 kDa

    • Amino acids

      1 to 1706

    • Additional sequence information

      ab179866 also contains the cyaC activator gene product (aa 1-185; MWt 20kDa) (UniProt P0A3I5).

Specifications

Our Abpromise guarantee covers the use of ab179866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Western blot

    SDS-PAGE

    ELISA

  • Form

    Liquid

  • Additional notes

    This protein is derived from a pathogenic organism (Bordatella pertussis) and may be involved in a disease process, consequently exposure may have adverse health effects

    The toxin can be inactivated by boiling for 30 min.

    LD50 for mouse is approximately 20 ng / mouse. No LD50 information for human is known.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 36.04% Urea, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • AC-HLY
    • ACT
    • bifunctional hemolysin-adenylate cyclase
    • cya
    • Cyclolysin
    see all

  • Cellular localization

    Secreted

Images

  • SDS-PAGE - Recombinant <em>B. pertussis</em> Adenylate Cyclase protein (ab179866)
    SDS-PAGE - Recombinant B. pertussis Adenylate Cyclase protein (ab179866)

    8% SDS-PAGE analysis of ab179866.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab179866? Please let us know so that we can cite the reference in this datasheet.

ab179866 has not yet been referenced specifically in any publications.

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