Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant Bacteriophage P1 Cre recombinase protein
Purity> 90 % SDS-PAGE.
ab134845 is chromatographically purified.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMASMTGGQQM GRGHHHHHHG NLYFQGGEFP PKKKRKVSNL LTVHQNLPAL PVDATSDEVR KNLMDMFRDR QAFSEHTWKM LLSVCRSWAA WCKLNNRKWF PAEPEDVRDY LLYLQARGLA VKTIQQHLGQ LNMLHRRSGL PRPSDSNAVS LVMRRIRKEN VDAGERAKQA LAFERTDFDQ VRSLMENSDR CQDIRNLAFL GIAYNTLLRI AEIARIRVKD ISRTDGGRML IHIGRTKTLV STAGVEKALS LGVTKLVERW ISVSGVADDP NNYLFCRVRK NGVAAPSATS QLSTRALEGI FEATHRLIYG AKDDSGQRYL AWSGHSARVG AARDMARAGV SIPEIMQAGG WTNVNIVMNY IRNLDSETGA MVRLLEDGDL EESGGGGSPG RRRRRRRRRR R
Amino acids2 to 343
Additional sequence informationN-terminal T7 tag/His/TEV cleavage site tag and C-terminal 11R tag.
Our Abpromise guarantee covers the use of ab134845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1. Protein intracellular transduction for in vitro Cre-loxP recombination.
2. Negative control for 11R-Tag human transcription factor mediated cell differentiation assay in vitro.
Functional Assay: Mix 1µg Cre-11R protein with 2ul ProFectinTM by following ProFectinTM manufacture suggested protocol, and incubate transfection mixture on 0.25 x106 293-LoxP-eGFP cell line / well (6 well plate) for 30 min in serum free medium, will achieve > 35% transduction efficiency. (Positive for GFP cells).
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.02% DTT, 0.32% Tris HCl, 0.58% Sodium chloride, 0.08% Potassium chloride, 10% Glycerol
- Cyclization recombinase
- Recombinase cre
RelevanceCre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab134845 has not yet been referenced specifically in any publications.