Recombinant Bacteriophage P1 Cre recombinase protein (ab134845)

Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Bacteriophage P1
    • Sequence

      MASMTGGQQM GRGHHHHHHG NLYFQGGEFP PKKKRKVSNL LTVHQNLPAL PVDATSDEVR KNLMDMFRDR QAFSEHTWKM LLSVCRSWAA WCKLNNRKWF PAEPEDVRDY LLYLQARGLA VKTIQQHLGQ LNMLHRRSGL PRPSDSNAVS LVMRRIRKEN VDAGERAKQA LAFERTDFDQ VRSLMENSDR CQDIRNLAFL GIAYNTLLRI AEIARIRVKD ISRTDGGRML IHIGRTKTLV STAGVEKALS LGVTKLVERW ISVSGVADDP NNYLFCRVRK NGVAAPSATS QLSTRALEGI FEATHRLIYG AKDDSGQRYL AWSGHSARVG AARDMARAGV SIPEIMQAGG WTNVNIVMNY IRNLDSETGA MVRLLEDGDL EESGGGGSPG RRRRRRRRRR R
    • Amino acids

      2 to 343
    • Additional sequence information

      C-terminal tag with an 11 arginine domain. (YP_006472)

Specifications

Our Abpromise guarantee covers the use of ab134845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity

    > 90 % SDS-PAGE.
    ab134845 is chromatographically purified.
  • Form

    Liquid
  • Additional notes

    Applications:

    1. Protein intracellular transduction for in vitro Cre-loxP recombination.

    2. Negative control for 11R-Tag human transcription factor mediated cell differentiation assay in vitro.

    Functional Assay: Mix 1µg Cre-11R protein with 2ul ProFectinTM by following ProFectinTM manufacture suggested protocol, and incubate transfection mixture on 0.25 x106 293-LoxP-eGFP cell line / well (6 well plate) for 30 min in serum free medium, will achieve > 35% transduction efficiency. (Positive for GFP cells).

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 0.58% Sodium chloride, 0.08% Potassium chloride, 10% Glycerol

General Info

  • Alternative names

    • Cre
    • Cyclization recombinase
    • Recombinase cre
  • Relevance

    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.

Images

  • Analysis of 0.25 x106 293-LoxP-eGFP report cell line with 1 µg ab134845. Cells were cultivated overnight before viewing GFP positive cells.

References

ab134845 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



This protein is quality tested with the following specifics:

1. Purity: > 90% by SDS-PAGE.

2. Functional Assay: Mix 1µg Cre recombinase protein with 2ul ProFectinTM by following manufacturer's suggested protocol, and incubate transfection mixture on 0.25 x106 293-LoxP-eGFP cell line / well (6 well plate) for 30 min in serum free medium. This will achieve > 35% transduction efficiency. (Positive for GFP cells).

Unfortunately, the percentage of transduction efficiency is not guaranteed by our Abpromise since this is highly dependent on the sytem/cells used.

I hope this information is helpful and wish you good luck for your research.

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