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Recombinant Bacteriophage P1 Cre recombinase protein (ab134845)

Submit a review Q&A (1)
Functional Studies - Recombinant bacteriophage p1 Cre recombinase protein (ab134845)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Suitable for: Functional Studies, SDS-PAGE

    Description

    • Product name

      Recombinant Bacteriophage P1 Cre recombinase protein

    • Purity

      > 90 % SDS-PAGE.
      ab134845 is chromatographically purified.

    • Expression system

      Escherichia coli

    • Accession

      P06956

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Bacteriophage P1

      • Sequence

        MASMTGGQQM GRGHHHHHHG NLYFQGGEFP PKKKRKVSNL LTVHQNLPAL PVDATSDEVR KNLMDMFRDR QAFSEHTWKM LLSVCRSWAA WCKLNNRKWF PAEPEDVRDY LLYLQARGLA VKTIQQHLGQ LNMLHRRSGL PRPSDSNAVS LVMRRIRKEN VDAGERAKQA LAFERTDFDQ VRSLMENSDR CQDIRNLAFL GIAYNTLLRI AEIARIRVKD ISRTDGGRML IHIGRTKTLV STAGVEKALS LGVTKLVERW ISVSGVADDP NNYLFCRVRK NGVAAPSATS QLSTRALEGI FEATHRLIYG AKDDSGQRYL AWSGHSARVG AARDMARAGV SIPEIMQAGG WTNVNIVMNY IRNLDSETGA MVRLLEDGDL EESGGGGSPG RRRRRRRRRR R

      • Amino acids

        2 to 343

      • Additional sequence information

        N-terminal T7 tag/His/TEV cleavage site tag and C-terminal 11R tag.

    Specifications

    Our Abpromise guarantee covers the use of ab134845 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

      Applications:

      1. Protein intracellular transduction for in vitro Cre-loxP recombination.

      2. Negative control for 11R-Tag human transcription factor mediated cell differentiation assay in vitro.

      Functional Assay: Mix 1µg Cre-11R protein with 2ul ProFectinTM by following ProFectinTM manufacture suggested protocol, and incubate transfection mixture on 0.25 x106 293-LoxP-eGFP cell line / well (6 well plate) for 30 min in serum free medium, will achieve > 35% transduction efficiency. (Positive for GFP cells).

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 0.58% Sodium chloride, 0.08% Potassium chloride, 10% Glycerol

    General Info

    • Alternative names

      • Cre
      • Cyclization recombinase
      • Recombinase cre

    • Relevance

      Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.

    Images

    • Functional Studies - Recombinant bacteriophage p1 Cre recombinase protein (ab134845)
      Functional Studies - Recombinant bacteriophage p1 Cre recombinase protein (ab134845)
      Analysis of 0.25 x106 293-LoxP-eGFP report cell line with 1 µg ab134845. Cells were cultivated overnight before viewing GFP positive cells.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab134845? Please let us know so that we can cite the reference in this datasheet.

    ab134845 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Submit a question

    Question


    We are interested in trying your cre recombinase tagged enzyme (ab134845), but first we would like to have some information about the efficiency of recombination in vivo.

    I've seen in the datasheet pictures of eGFP reporter cells and I was wondering if you could provide us with the entire study or at least the % of cells that underwent recombination. This is a crucial information for us because our present provider sells a Cre recombinase with 30% efficiency in vivo. If abcam's enzyme is better, we may change provider.

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    Answer



    This protein is quality tested with the following specifics:

    1. Purity: > 90% by SDS-PAGE.

    2. Functional Assay: Mix 1µg Cre recombinase protein with 2ul ProFectinTM by following manufacturer's suggested protocol, and incubate transfection mixture on 0.25 x106 293-LoxP-eGFP cell line / well (6 well plate) for 30 min in serum free medium. This will achieve > 35% transduction efficiency. (Positive for GFP cells).

    Unfortunately, the percentage of transduction efficiency is not guaranteed by our Abpromise since this is highly dependent on the sytem/cells used.

    I hope this information is helpful and wish you good luck for your research.

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