Question (10483) | Recombinant Calreticulin protein (ab15729)

Go to datasheet (ab15729)

Question

Hi: I bought calrituculin protein (cat :ab15729-100) from your company, it is solution 1mg/ml. What kind of solution in it? Does it contain Calcium or Zinc? Please let me know as soon as you can. Thank you!

Answer

My apologies for the delay in replying to your enquiry, we have just received the following information from the source of ab15729: "The protein is in PBS and we have not added any Ca or Zn to this solution. One may want to add a small amount of EGTA (0.05 uM) to chelate a residual Ca, if any. If there is any residual Ca present it would be in nanomolar concentration, a typical "contaminant" of any solution that does not contain a chelator. When we are interested in studying Ca effects on the protein we always add some EGTA to make sure we have full control of actual Ca concentration in the protein solution." I hope this information helps, please do not hesitate to contact us if you need further help,

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