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From which species is the Calreticulin protein obtained?
Asked on Oct 21 2004
Thank you for your enquiry. Here are some details regarding this protein: Recombinant calreticulin was expressed in E. coli and purified as His-tag protein. Full length cDNA encoding mature rabbit calreticulin was cloned into pBAD expression vector followed by expression in E. coli, cell lysis and affinity chromatography on Ni-column as described in (Guo et al., 2003). The protein has a C-terminal His-tag and has passed all biochemical tests in the lab to indicate that it is properly folded and functional. The best test for the quality of recombinant calreticulin involves limited proteolysis with trypsin as described in (Corbett et al., 2000). The final product is filter sterilized.
Answered on Oct 22 2004