Recombinant Catalase protein (Active) (ab219092)
Key features and details
- Expression system: Escherichia coli
- Purity: = 54% Ion Exchange Chromatography
- Active: Yes
- Suitable for: Functional Studies
Description
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Product name
Recombinant Catalase protein (Active)
See all Catalase proteins and peptides -
Biological activity
Specific activity: ≥ 8000 U/mg protein.
Unit definition: One unit is defined as the decomposition of 1μmol of H2O2 in 1 minute at 25°C at pH 7.0. The rate of disappearance of H2O2 is observed at 240 nm .
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Purity
= 54 % Ion Exchange Chromatography.
54% after ion exchange chromatography. Purity is batch dependent. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Serratia -
Predicted molecular weight
220 kDa -
Additional sequence information
From Psychrotolerant Bacteria. Four subunits. Each subunit is ~ 55 kDa.
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Specifications
Our Abpromise guarantee covers the use of ab219092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C.
Constituents: 0.61% Tris, 2.9% Sodium chloride, 85% Catalase
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in deionized water.
General Info
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Alternative names
- Cas1
- CAT
- CATA_HUMAN
see all -
Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
Sequence similarities
Belongs to the catalase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Peroxisome. - Information by UniProt
Images
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Functional Studies - Recombinant Catalase protein (Active) (ab219092)
pH dependence of ab219092. Activity was measured by monitoring pH from 3 to 11 at 25ºC.
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Functional Studies - Recombinant Catalase protein (Active) (ab219092)Temperature dependence of ab219092. Activity was measured by monitoring temperature from 20 to 80ºC using 50 mM potassium phosphate buffer (pH 7.0).
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Functional Studies - Recombinant Catalase protein (Active) (ab219092)Thermostability analysis of ab219092. Activity was monitored at 50°C using 50 mM potassium phosphate buffer (pH 7.0).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab219092 has been referenced in 1 publication.
- Park J et al. Cell segmentation-free inference of cell types from in situ transcriptomics data. Nat Commun 12:3545 (2021). PubMed: 34112806