Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Species

      Serratia
    • Molecular weight

      220 kDa
    • Additional sequence information

      From Psychrotolerant Bacteria. Four subunits. Each subunit is ~ 55 kDa.

Specifications

Our Abpromise guarantee covers the use of ab219092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity: ≥ 8000 U/mg protein.

    Unit definition: One unit is defined as the decomposition of 1μmol of H2O2 in 1 minute at  25°C at pH 7.0. The rate of disappearance of H2O2 is observed at 240 nm . 

  • Applications

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

    Constituents: 0.61% Tris, 2.9% Sodium chloride, 85% Catalase

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in deionized water.

General Info

  • Alternative names

    • Cas1
    • CAT
    • CATA_HUMAN
    • Catalase
    • Cs1
    • MGC138422
    • MGC138424
    see all
  • Function

    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in disease

    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similarities

    Belongs to the catalase family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Peroxisome.
  • Information by UniProt

Images

  • pH dependence of ab219092. Activity was measured by monitoring pH from 3 to 11 at 25ºC.

  • Temperature dependence of ab219092. Activity was measured by monitoring temperature from 20 to 80ºC using 50 mM potassium phosphate buffer (pH 7.0). 

  • Thermostability analysis of ab219092. Activity was monitored at 50°C using 50 mM potassium phosphate buffer (pH 7.0).

References

ab219092 has not yet been referenced specifically in any publications.

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