Recombinant chicken Growth Hormone protein (Active) (ab68386)

Description

  • Product name

    Recombinant chicken Growth Hormone protein (Active)
    See all Growth Hormone proteins and peptides
  • Purity

    > 99 % SDS-PAGE.
    >99% SEC-HPLC. ab68386 is purified by proprietary chromatographic techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Chicken
    • Sequence

      ATFPAMPLSN LFANAVLRAQ HLHLLAAETY KEFERTYIPE DQRYTNKNSQ AAFCYSETIP APTGKDDAQQ KSDMELLRFS LVLIQSWLTP VQYLSKVFTN NLVFGTSDRV FEKLKDLEEG IQALMRELED RSPRGPQLLR PTYDKFDIHL RNEDALLKNY GLLSCFKKDL HKVETYLKVM KCRRFGESNC TI
    • Predicted molecular weight

      22 kDa
    • Amino acids

      25 to 216
    • Additional sequence information

      Full length mature protein, without the signal peptide.

Specifications

Our Abpromise guarantee covers the use of ab68386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    HPLC

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Upon reconstitution at > 0.1 mg/ml and filter sterilization GH can be stored at 4°C for several weeks.

    Protein quantitation was carried out by two independent methods:
    1. UV spectroscopy at 280 nm using the absorbency value of 0.75 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 
    2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    Preservative: None.
    Constituents: 0.3% Sodium bicarbonate, pH 8.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

General Info

  • Alternative names

    • gH
    • GH-N
    • GH1
    • GHB5
    • GHN
    • Growth hormone
    • Growth hormone 1
    • Growth hormone B5
    • Growth hormone, normal
    • Growth hormone, pituitary
    • HG1
    • hGH-N
    • IGHD1B
    • Pituitary growth hormone
    • RNGHGP
    • SOMA_HUMAN
    • Somatotropin
    see all
  • Function

    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • Involvement in disease

    Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
    Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
  • Sequence similarities

    Belongs to the somatotropin/prolactin family.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab68386 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Answer

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We have one product in catalogue that could be suitable as per your requirements.

ab68386; https://www.abcam.com/Chicken-Growth-Hormone-protein-Active-ab68386.html

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