Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Chicken
    • Sequence

      EVNYRTVTINTEQKVSDVYNIEERLGSGKFGQVFRLVEKKTGKVWAGKFF KAYSAKEKENIRDEISIMNCLHHPKLVQCVDAFEEKANIVMVLEMVSGGE LFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVN KTGTSIKLIDFGLARRLESAGSLKVLFGTPEFVAPEVINYEPIGYETDMW SIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDF ISNLLKKDMKSRLNCTQCLQHPWLQKDTKNMEAKKLSK
    • Molecular weight

      211 kDa
    • Amino acids

      1435 to 1722
    • Tags

      His tag N-Terminus
    • Additional sequence information

      N-terminal 10xHis-SUMO-tagged and C-terminal MYC-tagged.

Specifications

Our Abpromise guarantee covers the use of ab235864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Purity

    > 90 % SDS-PAGE.

  • Form

    Liquid
  • Additional notes

    Previously labelled as Myosin light chain kinase.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Constituents: 50% Glycerol, Tris buffer

General Info

  • Alternative names

    • deglutamylated form
    • DKFZp686I10125
    • EC 2.7.11.18
    • FLJ12216
    • Kinase related protein
    • Kinase-related protein
    • KRP
    • MLCK
    • MLCK1
    • MLCK108
    • MLCK210
    • MSTP083
    • MYLK
    • MYLK_HUMAN
    • MYLK1
    • Myosin light chain kinase
    • Myosin light polypeptide kinase
    • OTTHUMP00000180642
    • OTTHUMP00000180643
    • smMLCK
    • smooth muscle
    • Smooth muscle myosin light chain kinase
    • Telokin
    see all
  • Function

    Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
  • Tissue specificity

    Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 fibronectin type-III domain.
    Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    MLCK is probably down-regulated by phosphorylation.
    The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
  • Information by UniProt

Images

  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel of ab235864.

References

ab235864 has not yet been referenced specifically in any publications.

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