Recombinant Chinese hamster P4HB protein (His tag) (ab241446)
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Description
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Product name
Recombinant Chinese hamster P4HB protein (His tag)
See all P4HB proteins and peptides -
Purity
> 85 % SDS-PAGE. -
Expression system
Yeast -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Chinese hamster -
Sequence
DAPEEEDNVLVLKKSNFAEALAAHNYLLVEFYAPWCGHCKALAPEYAKAA AKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYT AGREADDIVNWLKKRTGPAATTLSDTAAAETLIDSSEVAVIGFFKDVESD SAKQFLLAAEAVDDIPFGITSNSGVFSKYQLDKDGVVLFKKFDEGRNNFE GEVTKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSD YDGKLGNFKKAAEGFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRL ITLEEEMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQELPEDWDKQ PVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDH ENIIIAKMDSTANEVEAVKVHSFPTLKFFPATADRTVIDYNGERTLDGFK KFLESGGQDGAGDDDDVDLEEALEPDMEEDDDQKAVKDEL -
Predicted molecular weight
57 kDa including tags -
Amino acids
20 to 509 -
Tags
His tag N-Terminus -
Additional sequence information
This product is the mature full length protein from aa 20 to 509. The signal peptide is not included.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab241446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: Tris buffer, 50% Glycerol
General Info
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Alternative names
- Cellular thyroid hormone binding protein
- Cellular thyroid hormone-binding protein
- Collagen prolyl 4 hydroxylase beta
see all -
Function
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
Images
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SDS-PAGE - Recombinant Chinese hamster P4HB protein (His tag) (ab241446)(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab241446.
Datasheets and documents
References
ab241446 has not yet been referenced specifically in any publications.
