Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: Functional Studies
Product nameRecombinant Cow Enterokinase protein
See all Enterokinase proteins and peptides
Purity> 95 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthProtein fragment
Our Abpromise guarantee covers the use of ab60893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesThis product is supplied as 1 vial containing ~0.2units of protein.
This protein can be used for fusion protein cleavage. The rare 5 amino acid recognition sequence of the enterokinase cleavage site is incorporated into a tag with the sequence: N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C Cleavage by enterokinase removes the tag from the fusion protein.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.0222% Calcium chloride, 0.316% Tris HCl, 50% Glycerol, 1.16% Sodium chloride
- PRSS 7
FunctionResponsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
Tissue specificityIntestinal brush border.
Involvement in diseaseDefects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD) [MIM:226200]; a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 2 LDL-receptor class A domains.
Contains 1 MAM domain.
Contains 1 peptidase S1 domain.
Contains 1 SEA domain.
Contains 1 SRCR domain.
modificationsThe chains are derived from a single precursor that is cleaved by a trypsin-like protease.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab60893 has not yet been referenced specifically in any publications.