Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
Product nameRecombinant cow Hsc70 protein
See all Hsc70 proteins and peptides
Biological activityATPase Activity Assay: Positive
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab91596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesEndotoxin Concentration: < 50 EU/mg as determined by Limulus Amebocyte Lysate (LAL) gel clot assay
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.0308% DTT, 0.363% Tris, 0.0292% EDTA, 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Constitutive heat shock protein 70
- Epididymis luminal protein 33
FunctionActs as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
Sequence similaritiesBelongs to the heat shock protein 70 family.
DomainThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab91596 has not yet been referenced specifically in any publications.